Abstract
Twenty-eight nuclear microsatellites were isolated for the hispid pocket mouse, Chaetodipus hispidus, and characterized in 33 individuals from four genetically distinct groups throughout the geographic range of the species. Dinucleotide and trinucleotide microsatellite repeat motifs were screened, and of the 28 primer pairs created, eight amplified and were polymorphic. After correction for multiple tests, no loci deviated significantly from Hardy–Weinberg expectations. Working primers also were tested in other closely related species found within the subfamily Perognathinae. The microsatellite markers characterized in this study will be beneficial towards future population genetic research within the hispid pocket mouse and other Perognathinae rodents.
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Acknowledgments
We would like to thank J. Gold, S. Welborn, C. Nessner, and C. Rodriguez for their assistance throughout this research. Our thanks to the following institutions and curators for providing tissue samples: American Museum of Natural History, Angelo State Natural History Collections, Louisiana State University Museum of Natural Science, Natural Science Research Laboratory at the Museum of Texas Tech University, New Mexico Museum of Natural History, Sam Noble Oklahoma Museum of Natural History, University of Kansas Natural History Museum, University of New Mexico Museum of Southwestern Biology, and University of Nevada Las Vegas. This is publication number 1433 of the Texas Cooperative Wildlife Collection and number 215 of the Center for Biosystematics and Biodiversity, both at Texas A&M University.
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Andersen, J.J., Renshaw, M.A. & Light, J.E. Eight novel polymorphic microsatellites in the hispid pocket mouse (Chaetodipus hispidus) and cross-amplification in other Perognathinae species (Rodentia: Heteromyidae). Conservation Genet Resour 4, 1019–1021 (2012). https://doi.org/10.1007/s12686-012-9697-3
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DOI: https://doi.org/10.1007/s12686-012-9697-3