Conservation Genetics Resources

, Volume 4, Issue 4, pp 901–903 | Cite as

Characterization of nine microsatellite loci in the European polecat Mustela putorius

  • Claudio Ciofi
  • Chiara Natali
  • Paolo Agnelli
  • Emiliano Manzo
  • Paola Bartolommei
  • Roberto Cozzolino
Technical Note

Abstract

We developed a set of nine polymorphic microsatellite markers from a genomic library enriched for dinucleotide repeats in the European polecat Mustela putorius. Microsatellite loci amplification was tested on fresh tissues and museum samples collected over the last 40 years in central and northern Italy. Number of alleles per locus ranged from 3 to 7. Mean observed and expected heterozygosity were 0.48 and 0.61, respectively. This novel set of species-specific microsatellite loci will be particularly useful to assess fine patterns of genetic structure and degree of isolation of European polecat populations.

Keywords

European polecat Mustelidae Microsatellites Population genetics Museum samples 

References

  1. Anistoroaei R, Christensen K (2006) A test of mink microsatellite markers in the ferret: amplification and sequence comparisons. Hereditas 143:198–201PubMedCrossRefGoogle Scholar
  2. Blandford PRS (1987) Biology of the polecat Mustela putorius: a literature review. Mammal Rev 17:155–198CrossRefGoogle Scholar
  3. Ciofi C, Tzika AC, Natali C, Chelazzi G, Naziridis T, Milinkovitch MC (2009) Characterization of microsatellite loci in the European pond turtle Emys orbicularis. Mol Ecol Resour 9:189–191PubMedCrossRefGoogle Scholar
  4. Edwards KJ, Barker JHA, Daly A, Jones C, Karp A (1996) Microsatellite libraries enriched for several microsatellite sequences in plants. Biotechniques 20:758–760PubMedGoogle Scholar
  5. Genovesi P, De Marinis AM (2003) Mustela putorius (Linnaeus, 1758). In: Boitani L, Lovari S, Vigna Taglianti A (eds) Fauna d’Italia, Volume XXXVIII. Mammalia III, Carnivora - Artiodactyla. Il Sole 24 Ore Edagricole, Milano, pp 128–132Google Scholar
  6. Møller TB, Pertoldi C, Madsen AB et al (2004) Genetic variability in Danish polecats Mustela putorius as assessed by microsatellites. Wildlife Biol 10:25–33Google Scholar
  7. Pertoldi C, Breyne P, Cabria MT et al (2006) Genetic structure of the European polecat (Mustela putorius) and its implication for conservation strategies. J Zool 270:102–115Google Scholar
  8. Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106PubMedCrossRefGoogle Scholar
  9. Schretter C, Milinkovitch MC (2006) OLIGOFAKTORY: a visual tool for interactive oligonucleotide design. Bioinformatics 22:115–116PubMedCrossRefGoogle Scholar
  10. Sokal RR, Rohlf FJ (1994) Biometry: the principles and practice of statistics in biological research. Freeman, New YorkGoogle Scholar
  11. Untergasser A, Nijveen H, Rao X, Bisseling T, Geurts R, Leunissen JAM (2007) Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res 35:W71–W74PubMedCrossRefGoogle Scholar
  12. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  13. Wolsan M (1993) Phylogeny and classification of early European Mustelida (Mammalia, Carnivora). Acta Theriol 38:345–384Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Claudio Ciofi
    • 1
  • Chiara Natali
    • 1
  • Paolo Agnelli
    • 2
  • Emiliano Manzo
    • 3
  • Paola Bartolommei
    • 3
  • Roberto Cozzolino
    • 3
  1. 1.Molecular Ecology Laboratory, Department of Evolutionary BiologyUniversity of FlorenceFlorenceItaly
  2. 2.Florence Natural History MuseumUniversity of FlorenceFlorenceItaly
  3. 3.Fondazione EthoikosConvento dell’OsservanzaRadicondoliItaly

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