Abstract
Octopus vulgaris, Octopus cyanea and Callistoctopus ornatus may co-occur in near-shore shallow reef habitats across the Southwestern Indian Ocean. All three species are harvested, both by small vessels and individual spearfishers and are of considerable socio-economic importance. Phenotypic-based discrimination of the three species is unreliable and at present species-specific catch data are not collected, preventing evaluation of species specific fisheries effects. In light of increasing exploitation rates the development of an unambiguous means of species discrimination is required for sustainable management and conservation of species and intraspecific stocks. In this study, a simple, cost effective, molecular technique to differentiate the three species is described.
References
Guard M, Mgaya YD (2002) The artisanal fishery for Octopus cyanea Gray, 1849, in Tanzania. Ambio 31:528–536
Guerra A, Roura A, González AF, Pascual S, Cherel Y, Perez-Losada M (2010) Morphological and genetic evidence that Octopus vulgaris Cuvier, 1797 inhabits Amsterdam and Saint Paul Islands (southern Indian Ocean). ICES J Mar Sci 67:1401–1407
Hall TA (1999) Bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nuclear Acids Symp Ser 41:95–98
Norman MD (1991) Octopus cyanea Gray, 1849 (Mollusca, Cephalopoda) in Australian Waters—description, distribution and taxonomy. B Mar Sci 49:20–38
Van Heukelem WF (1973) Growth and life span of Octopus cyanea mollusca cephalopoda. J Zool (London) 169:299–315
Vincze T, Posfai J, Roberts RJ (2003) NEBcutter: a program to cleave DNA with restriction enzymes. Nucleic Acids Res 31:3688–3691
Wells MJ, Wells J (1970) Observations on the feeding, growth rate and habits of newly settled Octopus cyanea. J Zool 161:65–74
Winnepenninckx B, Backeljau T, De Wachter R (1993) Extraction of high molecular weight DNA from molluscs. Trends Genet 9:407
Yarnell JL (1969) Aspects of the behaviour of Octopus cyanea. Anim Behav 17:747–754
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Taylor, A.L., McKeown, N.J. & Shaw, P.W. Molecular identification of three co-occurring and easily misidentified octopus species using PCR–RFLP techniques. Conservation Genet Resour 4, 885–887 (2012). https://doi.org/10.1007/s12686-012-9665-y
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DOI: https://doi.org/10.1007/s12686-012-9665-y