Rapid isolation of 14 microsatellite markers for Van Diemen’s siphon limpet Siphonaria diemenensis
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Siphon limpets are an ecologically important component of the intertidal zone of Australia’s temperate coast. Using 1/16 of a shotgun pyrosequencing plate, a set of 14 microsatellites was developed for the Van Diemen’s siphon limpet (Siphonaria diemenensis). From 2,744 sequences that contained putative microsatellite motifs, 18 were selected for primer design and tested for amplification. Fourteen microsatellite loci were successfully genotyped in 48 specimens of S. diemenensis from two localities. The number of alleles per locus varied from 5 to 35 (mean = 14.6) and the observed heterozygosity ranged from 0.53 to 0.90 (mean = 0.77). Only one locus showed significant deviation from Hardy–Weinberg equilibrium, probably due to null alleles. No linkage disequilibrium between pairs of loci was detected. Connectivity and seascape genetic studies on siphon limpets using the markers reported here should provide important information for management of temperate Australian marine biodiversity.
KeywordsSeascape genetics Marine connectivity Population genetics MPA Mollusc Phylogeography
Funding for this study was provided by the Australian Research Council (DP110101275 to Beheregaray, Möller & Waters). We thank Minami Sasaki and Seraina Bracamonte for laboratory assistance. This article is publication #46 of the Molecular Ecology Group for Marine Research (MEGMAR).
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