Skip to main content

Development and characterization of sixteen microsatellite markers for the federally endangered species: Leptodea leptodon (Bivalvia: Unionidae) using paired-end Illumina shotgun sequencing

Abstract

We isolated and characterized a total of 16 microsatellite loci in Leptodea leptodon. Loci were screened in 24 individuals from across the species current range in Missouri. The number of alleles per locus ranged from 3 to 17, observed heterozygosity ranged from 0.208 to 1.000, and the probability of identity values ranged from 0.014 to 0.67. These new loci will be used for conducting investigations into the genetic structure and diversity of extant populations of this federally endangered species.

This is a preview of subscription content, access via your institution.

References

  1. Castoe TA, Poole AW, de Koning APJ, Jones KL, Tomback DF, Oyler-McCance SJ, Fike JA, Lance SL, Streicher JW, Smith EN, Pollack DD (2012) Rapid microsatellite identification from Illumina paired-end genomic sequencing in two birds and a snake. PLoS ONE 7(2):e30953

    PubMed  Article  CAS  Google Scholar 

  2. DeWoody AJ, Schupp J, Kenefic L et al (2004) Universal method for producing ROX-labeled size standards suitable for automated genotyping. Biotechniques 37:348–350

    PubMed  CAS  Google Scholar 

  3. Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991) ‘Touchdown’ PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19:4008

    PubMed  Article  CAS  Google Scholar 

  4. Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  5. Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Res 8:103–106

    Article  Google Scholar 

  6. U.S. Fish and Wildlife Service (2001) Endangered and threatened wildlife and plants; review of plant and animal species that are candidates or proposed for listing as endangered or threatened, annual notice of findings on recycled petitions, and annual description of progress on listing actions; proposed rule. Fed Regist 66:54808–54832

    Google Scholar 

  7. U.S. Fish and Wildlife Service (2010) Scaleshell mussel recovery plan (Leptodea leptodon). U.S. Fish and Wildlife Service, Fort Snelling, pp 118

    Google Scholar 

Download references

Acknowledgments

The samples used to generate these data were collected under USFWS permit # TE48832A-0. Funds for development of these microsatellite loci were provided by a grant from the Missouri Department of Conservation. Manuscript preparation was partially supported by the DOE under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Kevin J. Roe.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

O’Bryhim, J., Chong, J.P., Lance, S.L. et al. Development and characterization of sixteen microsatellite markers for the federally endangered species: Leptodea leptodon (Bivalvia: Unionidae) using paired-end Illumina shotgun sequencing. Conservation Genet Resour 4, 787–789 (2012). https://doi.org/10.1007/s12686-012-9644-3

Download citation

Keywords

  • Leptodea
  • Microsatellite
  • PAL_FINDER
  • PCR primers
  • SSR