Abstract
We describe isolation and characterization of 20 polymorphic tetranucleotide loci from the green turtle (Chelonia mydas). We identified an average of 12.5 alleles per locus based on screening of 31 individuals foraging in the Indian River Lagoon, Florida, USA. Observed heterozygosity ranged from 0.53 to 1.00, with a mean of 0.85. This microsatellite suite has a combined non-exclusion probability of identity of 2.45 × 10–28. These markers will complement those already available for green turtles in individual-based as well as population level genetic analyses.
References
Bagley DA (2003) Characterizing juvenile green turtles, (Chelonia mydas), from three east central Florida developmental habitats. Masters thesis. University of Central Florida, Orlando, Florida
Bowen BW, Karl SA (2007) Population genetics and phylogeography of sea turtles. Mol Ecol 16:4886–4907
Chaloupka M, Bjorndal K, Balazas G, Bolten A, Ehrhart L, Limpus C, Suganuma H, Troëng S, Yamaguchi M (2008) Encouraging outlook for recovery of a once severely exploited marine megaherbivore. Glob Ecol Biogeogr 17:297–304
Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991) ‘Touchdown’ PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19:4008
Dutton P, Frey A (2009) Characterization of polymorphic microsatellite markers from the green turtle (Chelonia mydas). Mol Ecol Resour 9:354–356
Faircloth BC (2006) GMCONVERT: file conversion for GENEMAPPER output files. Mol Ecol Notes 6:968–970
Faircloth B (2008) MSTACOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94
FitzSimmons NN, Moritz C, Moore SS (1995) Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution. Mol Biol Evol 12:432–440
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. In: Zimmer EA, Roalson EH (eds) Molecular evolution: producing the biochemical data, Part B. Academic Press, San Diego
Jackson J (1997) Reefs since Columbus. Coral Reefs 16:S23–S32
Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106
McClenachan L, Jackson JBC, Newman MJH (2006) Conservation implications of historic sea turtle nesting beach loss. Front Ecol Environ 4:290–296
Parsons J (1962) The green turtle and man. University of Florida Press, Gainesville
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Roberts M, Schwartz T, Karl S (2004) Global population genetic structure and male-mediated gene flow in the green sea turtle (Chelonia mydas): analysis of microsatellite loci. Genetics 166:1857–1870
Rosen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totowa, pp 365–386
Shamblin BM, Faircloth BC, Dodd M, Wood-Jones A, Castleberry SB, Carroll JP, Nairn CJ (2007) Tetranculeotide microsatellites from the loggerhead sea turtle (Caretta caretta). Mol Ecol Notes 7:784–787
Shamblin BM, Faircloth BC, Dodd MG, Bagley DA, Ehrhart LM, Dutton PH, Frey A, Nairn CJ (2009) Tetranucleotide microsatellites from the loggerhead sea turtle (Caretta caretta) and their cross-amplification in other marine turtle species. Conserv Genet 10:577–580
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Shamblin, B.M., Berry, B.E., Lennon, D.M. et al. Tetranucleotide microsatellite loci from the endangered green turtle (Chelonia mydas). Conservation Genet Resour 4, 783–785 (2012). https://doi.org/10.1007/s12686-012-9643-4
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DOI: https://doi.org/10.1007/s12686-012-9643-4