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Characterization of 10 novel microsatellite loci and cross-amplification of two loci in the snapping turtle (Chelydra serpentina)

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Abstract

We use 454 (“shotgun”) sequencing to obtain a partial genomic library for the snapping turtle (Chelydra serpentina). We characterize ten microsatellite loci from these sequences and test cross-amplification of loci originally developed for the alligator snapping turtle (Macrochelys temminckii). We genotype 127 individuals from Ontario at twelve loci. The number of alleles per locus ranges from 1 to 14; heterozygosity ranges from 0.157 to 0.850. These loci will be used to study population genetic structure in this long-lived reptile and may cross-amplify in two closely related species.

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References

  • Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of nontemplated nucleotide addition by tag DNA polymerase: primer modifications that facilitate genotyping. BioTech 20:1004–1010

    CAS  Google Scholar 

  • Ernst C, Lovich J (2009) Turtles of the United States and Canada, 2nd edn. Johns Hopkins University Press, Baltimore

    Google Scholar 

  • Faircloth BC (2008) msatcommander: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94

    Article  PubMed  CAS  Google Scholar 

  • Hackler JC, van den Bussche RAV, Leslie DM (2006) Characterization of microsatellite DNA markers for the alligator snapping turtle, Macrochelys temminckii. Mol Ecol Notes 7:474–476

    Article  Google Scholar 

  • King TL, Julian SE (2004) Conservation of microsatellite DNA flanking sequences across 13 Emydid genera assayed with novel bog turtle (Glyptemys muhlenbergii) loci. Conserv Genet 5:719–725

    Article  Google Scholar 

  • Peakall R, Smouse PE (2006) Genalex 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  • Phillips CA, Dimmick WW, Carr JL (1996) Conservation genetics of the common snapping turtle (Chelydra serpentina). Conserv Biol 10:397–405

    Article  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386. Available at http://fokker.wi.mit.edu/primer3/

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning—a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

    Article  PubMed  CAS  Google Scholar 

  • Smith LM, Burgoyne LA (2004) Collecting, archiving and processing DNA from wildlife samples using FTA® databasing paper. BMC Ecol 4:4. http://www.biomedcentral.com/1472-6785/4/4

  • van Dijk PP (2011) Chelydra serpentina. In: IUCN 2011. IUCN red list of threatened species. Version 2011.2. www.iucnredlist.org. Downloaded on 12 December 2011

Download references

Acknowledgments

This project was supported by a Canada Collection grant from Wildlife Preservation Canada (WPC) to C.D. and a National Science and Engineering Research Council (NSERC) Discovery Grant (A3148) to R.W.M. Genotyping costs were offset by the generous assistance of the Schad Foundation. C.D. and I.D. are funded by Canada Graduate Scholarships from NSERC; A.L. is funded by WPC through a Science Horizons grant from Environment Canada. We thank Dr. C. Kyle, E. Kerr and M. Harnden at the NRDPFC, Trent University, for assistance with 454 sequencing. Sample collection was conducted with the permission of the Government of Ontario and Ontario Parks and followed Animal Use Protocol 2010-14 (Royal Ontario Museum).

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Authors and Affiliations

  1. Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2, Canada

    Christina M. Davy, Ida M. Conflitti & Robert W. Murphy

  2. Department of Natural History, Royal Ontario Museum, 100 Queen’s Park, Toronto, ON, M5S 2C6, Canada

    Christina M. Davy, Ida M. Conflitti & Robert W. Murphy

  3. Wildlife Preservation Canada, RR #5, 5420 Highway 6 North, Guelph, ON, N1H 6J2, Canada

    Ashley E. Leifso

Authors
  1. Christina M. Davy
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  2. Ashley E. Leifso
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  3. Ida M. Conflitti
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  4. Robert W. Murphy
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Correspondence to Christina M. Davy.

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Davy, C.M., Leifso, A.E., Conflitti, I.M. et al. Characterization of 10 novel microsatellite loci and cross-amplification of two loci in the snapping turtle (Chelydra serpentina). Conservation Genet Resour 4, 695–698 (2012). https://doi.org/10.1007/s12686-012-9624-7

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  • DOI: https://doi.org/10.1007/s12686-012-9624-7

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