Abstract
We present a set of nuclear DNA SNPs useful for discriminating native Lake Biwa and introduced “Eurasian” strains of common carp, and further, for detecting their hybridization. Such SNPs were searched around short tandem repeat (STR) regions to be used later as HapSTR markers. A total of 62 microsatellite locus were screened with PCR and direct sequencing. The resultant nucleotide sequences from typical Lake Biwa (n = 30) and “Eurasian” (n = 24) specimens indicated that a total of 21 SNPs distributed across 7 loci would be useful for the above-mentioned purpose. All of the SNPs can be genotyped by direct sequencing, simultaneously with STR polymorphisms.
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Acknowledgments
We thank the following institution and individuals for their help in collecting the specimens: Shiga Prefectural Fisheries Experiment Station, Martin Hulak, Kei’ichiro Iguchi, Katsutoshi Watanabe, Chunguang Zhang, Katsuki Nakai and Hiroshi Senou. This research was partly supported by the Ministry of Education, Science, Sports, and Culture, Grant-in-Aid for young Scientists (B) 18780142 and 21710242, Grant-in-Aid for Scientific Research (C) 18510209 and (B) 19405011.
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Mabuchi, K., Song, H.Y., Takeshima, H. et al. A set of SNPs near or within STR regions useful for discriminating native Lake Biwa and introduced “Eurasian” strains of common carp. Conservation Genet Resour 4, 649–652 (2012). https://doi.org/10.1007/s12686-012-9613-x
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DOI: https://doi.org/10.1007/s12686-012-9613-x