Eight anonymous nuclear loci for the squamate antbird (Myrmeciza squamosa), cross-amplifiable in other species of typical antbirds (Aves, Thamnophilidae)


Typical antbirds (~209 species) represent a diverse radiation of Neotropical birds that includes many species of conservation concern. Here we present eight anonymous nuclear loci designed for the squamate antbird Myrmeciza squamosa, a species endemic to the Atlantic Forest of Brazil. We also show that those anonymous nuclear loci are amplifiable in a number of other typical antbird species from related genera (Myrmeciza, Percnostola, Gymnocichla, Myrmoborus, Pyriglena and Formicivora), including three threatened species (Myrmeciza ruficauda, Formicivora littoralis, and Pyriglena atra). Those markers will be useful not only to help management of threatened species of typical antbirds, but also to explore their evolutionary histories, both at intra and interspecific levels.

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  1. Balakrishnan CN, Lee JY, Edwards SV (2010) Phylogeography and phylogenetics in the nuclear age. In: Grant PR, Grant BR (eds) From field observations to mechanisms. A program in evolutionary biology. Princeton University Press, Priceton, pp 65–88

    Google Scholar 

  2. IUCN (2011) IUCN red list of threatened species. Version 2011.2. http://www.iucnredlist.org. Accessed on 10 Nov 2011

  3. Jennings WB, Edwards SV (2005) Speciational history of Australian grass finches (Poephila) inferred from thirty gene trees. Evolution 59(9):2033–2047

    PubMed  CAS  Google Scholar 

  4. Lee JY, Edwards SV (2008) Divergence across Australia’s Carpentarian barrier: statistical phylogeography of the red-backed fairy wren (Malurus melanocephalus). Evolution 62(12):3117–3134

    PubMed  Article  Google Scholar 

  5. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Towana, pp 365–386

    Google Scholar 

  6. Zimmer KJ, Isler M (2003) Family Thamnophilidae (typical antbirds). In: del Hoyo JM, Elliot A, Christie DA (eds) Handbook of the birds of the world, vol 8., Broadbills to tapaculosLynx Edicións, Barcelona, pp 448–681

    Google Scholar 

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Many thanks to Robb Brumfield and Donna Dittman (LSUMNS) for providing the M. squamosa tissue, Ingrid Soltero, June Yong Lee and Chris Hamm for help with protocol optimization, and Marcos M. Coelho for performing most of the cross-amplification tests. FRA and CYM received funds from FAPESP, CAPES and CNPq. SVE received funds from NSF.

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Correspondence to Fábio Raposo do Amaral.

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do Amaral, F.R., Edwards, S.V. & Miyaki, C.Y. Eight anonymous nuclear loci for the squamate antbird (Myrmeciza squamosa), cross-amplifiable in other species of typical antbirds (Aves, Thamnophilidae). Conservation Genet Resour 4, 645–647 (2012). https://doi.org/10.1007/s12686-012-9612-y

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  • Anonymous nuclear loci
  • Typical antbirds
  • Thamnophilidae
  • Myrmeciza