Skip to main content

Advertisement

SpringerLink
Log in

Rapid and reliable inference of mitochondrial phylogroups among Watersipora species, an invasive group of ship-fouling species (Bryozoa, Cheilostomata)

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Tracking biological invasion is an essential part of conservation, and identifying invasive species quickly following an introduction can be imperative. Invasive organisms are often difficult to distinguish visually, which can delay appropriate response measures. We have designed a multiplex-PCR system to effectively distinguish colonies of divergent cytochrome oxidase subunit 1 (COI) sequence clades in the bryozoan genus Watersipora, a genus with invasive species which are aggressive foulers of ship hulls, to understand small-scale invasion patterns and the ecology of lineages of different geographic origins. The identification of previously defined COI lineage groups is conducted using PCR reactions containing five primers. Lineage-specific fragment lengths were produced with 100% reliability, suggesting the assay will offer a rapid and economical means of studying invasion dynamics and the role of adaptation in this rapidly invading species complex.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Dafforn KA, Glasby TM, Johnston EL (2008) Differential effects of tributyltin and copper antifoulants on recruitment of non-indigenous species. Biofouling 24:23–33

    Article  PubMed  CAS  Google Scholar 

  • Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3:294–299

    PubMed  CAS  Google Scholar 

  • Geller J, Mackie JA, Schroeder G, Gerhinger D (2008) Distribution of highly invasive bryozoans belonging to a cryptic species complex in the genus Watersipora determined by DNA sequences, Final Report to California Department of Fish and Game, Moss Landing Marine Laboratories, CA, 40 pp

  • Mackie JA, Keough MJ, Christidis L (2006) Invasion patterns inferred from cytochrome oxidase I sequences in three bryozoans; Bugula neritina, Watersipora subtorquata, and Watersipora arcuata. Mar Biol 149:285–295

    Article  CAS  Google Scholar 

  • Owczarzy R, You Y, Moreira BG, Manthey JA, Huang L, Bahlke MA, Walder JA (2004) Effects of sodium ions on DNA duplex oligomers: improved predictions of melting temperatures. Biochemistry 43:3537–3554

    Article  PubMed  CAS  Google Scholar 

  • Ryland JS, De Blauwe H, Lord R, Mackie JA (2009) Recent discoveries of alien Watersipora (Bryozoa) in Western Europe, with redescriptions of species. Zootaxa 2093:43–59

Download references

Acknowledgments

We thank Anthony Baker (Humboldt State University Ecological Genetic Facility) and Reginald Blackwell for assistance. We thank the California State University Council on Ocean Affairs, Science and Technology (COAST) consortium and the National Science Foundation (NSF award #1061695) for financial support.

Author information

Authors and Affiliations

  1. University of Hawaii at Mānoa, 2538 McCarthy Mall, Edm. 152, Honolulu, HI, 96821, USA

    Áki J. Láruson

  2. Humboldt State University, 1 Harpst St., Arcata, CA, 95521, USA

    Sean F. Craig & Kirk J. Messer

  3. San José State University, One Washington Square, San José, CA, 95192, USA

    Joshua A. Mackie

Authors
  1. Áki J. Láruson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sean F. Craig
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kirk J. Messer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Joshua A. Mackie
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Joshua A. Mackie.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Láruson, Á.J., Craig, S.F., Messer, K.J. et al. Rapid and reliable inference of mitochondrial phylogroups among Watersipora species, an invasive group of ship-fouling species (Bryozoa, Cheilostomata). Conservation Genet Resour 4, 617–619 (2012). https://doi.org/10.1007/s12686-012-9606-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-012-9606-9

Keywords

Search

Navigation