Conservation Genetics Resources

, Volume 4, Issue 3, pp 571–573 | Cite as

Development of eight microsatellite loci from the endangered huemul (Hippocamelus bisulcus) and cross-species amplification in six other ungulate species

  • Aaron B. A. Shafer
  • Paulo Corti
  • David W. Coltman
  • Juan Carlos Marín
  • Corey S. DavisEmail author
Technical Note


We developed eight polymorphic microsatellite loci for the huemul (Hippocamelus bisulcus) and tested for cross-species amplification in six other ungulate species. Microsatellite loci were typed in 58 individuals from the Lago Cochrane National Reserve, Chile. Two to five alleles were observed per locus (mean 3.4) and observed heterozygosity ranged from 0.29 to 0.60 (mean 0.53). One to five loci amplified in other ungulate species. These markers will be used to refine studies of population structure and allow for more accurate pedigree reconstruction in the huemul.


Microsatellites Huemul Andean deer Hippocamelus bisulcus 



Microsatellite identification and primer design were part of the Biology 592 class project at the University of Alberta; specifically, we wish to thank Kelsey Bernard, Heather Bird and Wentong Long. We thank the Department of Biological Sciences the Molecular Biology Facility (MBSU) at the University of Alberta for their support. Thanks to all those involved in sample collection (listed in Corti et al. 2011).


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Aaron B. A. Shafer
    • 1
  • Paulo Corti
    • 2
  • David W. Coltman
    • 1
  • Juan Carlos Marín
    • 3
  • Corey S. Davis
    • 1
    Email author
  1. 1.Department of Biological SciencesUniversity of AlbertaEdmontonCanada
  2. 2.Instituto de Ciencia Animal, Programa de Investigación Aplicada en Fauna Silvestre, Facultad de Ciencias VeterinariasUniversidad Austral de ChileValdiviaChile
  3. 3.Departamento de Ciencias Básicas, Facultad de CienciasUniversidad del Bío-BíoChillánChile

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