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Conservation Genetics Resources

, Volume 4, Issue 3, pp 571–573 | Cite as

Development of eight microsatellite loci from the endangered huemul (Hippocamelus bisulcus) and cross-species amplification in six other ungulate species

  • Aaron B. A. Shafer
  • Paulo Corti
  • David W. Coltman
  • Juan Carlos Marín
  • Corey S. DavisEmail author
Technical Note
  • 128 Downloads

Abstract

We developed eight polymorphic microsatellite loci for the huemul (Hippocamelus bisulcus) and tested for cross-species amplification in six other ungulate species. Microsatellite loci were typed in 58 individuals from the Lago Cochrane National Reserve, Chile. Two to five alleles were observed per locus (mean 3.4) and observed heterozygosity ranged from 0.29 to 0.60 (mean 0.53). One to five loci amplified in other ungulate species. These markers will be used to refine studies of population structure and allow for more accurate pedigree reconstruction in the huemul.

Keywords

Microsatellites Huemul Andean deer Hippocamelus bisulcus 

Notes

Acknowledgments

Microsatellite identification and primer design were part of the Biology 592 class project at the University of Alberta; specifically, we wish to thank Kelsey Bernard, Heather Bird and Wentong Long. We thank the Department of Biological Sciences the Molecular Biology Facility (MBSU) at the University of Alberta for their support. Thanks to all those involved in sample collection (listed in Corti et al. 2011).

References

  1. Corti P, Shafer ABA, Coltman DW, Festa-Bianchet M (2011) Past bottlenecks and current population fragmentation of endangered huemul deer (Hippocamelus bisulcus): implications for preservation of genetic diversity. Conserv Genet 12:119–128CrossRefGoogle Scholar
  2. Engelke DR, Krikos A, Bruck ME, Ginsburg D (1990) Purification of Thermus aquaticus DNA polymerase expressed in Escherischia coli. Anal Biochem 191:396–400PubMedCrossRefGoogle Scholar
  3. Hamilton MB, Pincus EL, DiFiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. BioTechniques 27:500–507PubMedGoogle Scholar
  4. Jiménez J, Guineo G, Corti P, Smith JA, Flueck W, Vila A, Gizejewski Z, Gill R, McShea B, Geist V. (2008) Hippocamelus bisulcus. In: IUCN 2011. IUCN Red List of Threatened SpeciesGoogle Scholar
  5. Park SDE (2001) Trypanotolerance in West African cattle and the population genetic effects of selection. Dissertation, University of DublinGoogle Scholar
  6. Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Res 8:103–106CrossRefGoogle Scholar
  7. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–396Google Scholar
  8. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234PubMedCrossRefGoogle Scholar
  9. van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Aaron B. A. Shafer
    • 1
  • Paulo Corti
    • 2
  • David W. Coltman
    • 1
  • Juan Carlos Marín
    • 3
  • Corey S. Davis
    • 1
    Email author
  1. 1.Department of Biological SciencesUniversity of AlbertaEdmontonCanada
  2. 2.Instituto de Ciencia Animal, Programa de Investigación Aplicada en Fauna Silvestre, Facultad de Ciencias VeterinariasUniversidad Austral de ChileValdiviaChile
  3. 3.Departamento de Ciencias Básicas, Facultad de CienciasUniversidad del Bío-BíoChillánChile

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