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Development and characterization of tetranucleotide microsatellite loci for the American alligator (Alligator mississippiensis)

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Abstract

We isolated and characterized 17 tetranucleotide microsatellite loci in the American alligator, Alligator mississippiensis. Loci were screened across 27 individuals from one population and shown to be polymorphic with the number of alleles per locus ranging from 2 to 12. Polymorphic information content ranged from 0.2 to 0.85, and observed heterozygosity ranged from 0.185 to 0.889. One locus showed significant deviation from Hardy–Weinberg equilibrium, and one pair of loci showed evidence of linkage.

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Acknowledgments

This material is based upon work supported by the U.S. Department of Energy, through Financial Assistance Award No. DE-FC09-07SR22506 to the University of Georgia Research Foundation.

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Authors and Affiliations

  1. Savannah River Ecology Laboratory, P.O. Drawer E, Aiken, SC, 29802, USA

    Amanda L. Subalusky, Nancy A. Schable, Jessica Osborne & Travis C. Glenn

  2. Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, 06520, USA

    Amanda L. Subalusky & Ryan C. Garrick

  3. Department of Environmental Health Science, University of Georgia, Athens, GA, 30602, USA

    Travis C. Glenn

Authors
  1. Amanda L. Subalusky
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  2. Ryan C. Garrick
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  3. Nancy A. Schable
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  4. Jessica Osborne
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  5. Travis C. Glenn
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Corresponding author

Correspondence to Amanda L. Subalusky.

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Disclaimer: This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof.

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Subalusky, A.L., Garrick, R.C., Schable, N.A. et al. Development and characterization of tetranucleotide microsatellite loci for the American alligator (Alligator mississippiensis). Conservation Genet Resour 4, 567–570 (2012). https://doi.org/10.1007/s12686-011-9593-2

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  • DOI: https://doi.org/10.1007/s12686-011-9593-2

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