Fourteen new microsatellite markers for the Visayan tarictic hornbill (Penelopides panini) and their cross-species applicability among other endangered Philippine hornbills
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Fourteen microsatellite markers were isolated and characterized for the endangered Visayan tarictic hornbill (Penelopides panini, Aves: Bucerotidae). In an analysis of 76 individuals, the number of alleles per locus varied from one to 12. Expected and observed heterozygosity ranged from 0.00 to 0.87 and from 0.00 to 0.89, respectively. All primers also amplify microsatellite loci in Luzon tarictic hornbill (Penelopides manillae), Mindanao tarictic hornbill (Penelopides affinis), the critically endangered Walden’s hornbill (Aceros waldeni) and the near-threatened writhed hornbill (Aceros leucocephalus). Two loci which are monomorphic in P. panini were found polymorphic in at least one of the other species. These 14 new microsatellite markers specifically developed for two genera of Philippine hornbills, in combination with those already available for the hornbill genera Buceros and Bucorvus, comprise a reasonable number of loci to genetically analyse wild and captive populations of these and probably other related, often endangered hornbills.
KeywordsAceros Bucerotidae Cross-species amplification Microsatellites Penelopides Philippine hornbills
We thank Prof. Dr. Eberhard Curio for logistical support and helpful comments on the manuscript. Dr. Enrique Sanchez, Lucia L. Lastimoza, Antonio M. de Dios, Roberto P. Puentespina, Joaquin Gaw, Gerardo Ledesma, and José Bouffard kindly provided access to captive hornbills. The Protected Areas and Wildlife Bureau of the DENR (Director Dr. Mundita Lim) provided collecting, transport and export permits. CITES permits were obtained from Bundesamt für Naturschutz. Svenja Sammler is funded by Studienstiftung des Deutschen Volkes. Additional funds were provided by the University of Potsdam. This is publication no 84 of the Panay Eco-Social Conservation Project (PanayCon, formerly PESCP).
- BirdLife International (2011) IUCN Red List for birds. http://www.birdlife.org