Isolation and characterization of 55 novel microsatellite markers for the pink-footed goose (Anser brachyrhynchus)

Abstract

Here we present 55 new microsatellite markers isolated from the pink-footed goose (Anser brachyrhynchus), using next generation sequencing. Of these, 44 markers were found to be polymorphic, and the number of alleles ranged from two to ten with a mean of 4.23 alleles per locus. Mean observed heterozygosity was 0.44, and 33 polymorphic markers were in Hardy–Weinberg equilibrium in our sample population. These newly developed molecular markers could prove to be a useful tool for further studies of pink-footed goose populations.

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Acknowledgments

We thank Minna Ruokonen (University of Oulu) for extracting the DNA and Marika Karjalainen for help in the laboratory. Permits for catching the birds and DNA sampling were issued from the Governor of Svalbard and the Copenhagen Bird Ringing Centre to JM. This study was founded by a grant from the Academy of Finland to PG (grant no. 1123565).

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Correspondence to Phillip Gienapp.

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Noreikiene, K., Teacher, A.G.F., Madsen, J. et al. Isolation and characterization of 55 novel microsatellite markers for the pink-footed goose (Anser brachyrhynchus). Conservation Genet Resour 4, 423–428 (2012). https://doi.org/10.1007/s12686-011-9565-6

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Keywords

  • Anser
  • Pink-footed goose
  • Co-dominant markers
  • Microsatellite
  • Next generation sequencing