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First microsatellite loci for spectacled salamanders (Salamandrina perspicillata and S. terdigitata) endemic to the Apennine peninsula

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Abstract

We describe ten microsatellite loci isolated for the oldest lineage of extant salamandrids, the spectacled salamanders (Salamandrina), a genus of terrestrial salamanders endemic to the Apennine peninsula (Italy). Primers were tested in 24 individuals per species, the northern species (Salamandrina perspicillata) and the southern species (Salamandrina terdigitata). All loci were polymorphic in S. perspicillata with number of alleles ranging from three to eleven and observed heterozygosity ranging from 0.25 to 0.83. In S. terdigitata eight loci were polymorphic, the number of alleles ranged from two to nine, and observed heterozygosity from 0.04 to 0.92.

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References

  • Canestrelli D, Zangari F, Nascetti G (2006) Genetic evidence for two distinct species within the Italian endemic Salamandrina terdigitata (Bonnaterre, 1789) (Amphibia: Urodela: Salamandridae). Herpetol J 16:221–227

    Google Scholar 

  • Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Res 10:564–567

    Article  Google Scholar 

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222

    Article  PubMed  CAS  Google Scholar 

  • Hauswaldt JS, Fuessel J, Guenther J, Steinfartz S (2008) Eight new tetranucleotide microsatellite loci for the agile frog (Rana dalmatina). Mol Ecol Resour 8:1457–1459

    Article  PubMed  CAS  Google Scholar 

  • Hauswaldt JS, Angelini C, Pollok A, Steinfartz S (2011) Hybridization of two ancient salamander lineages: molecular evidence for endemic spectacled salamanders (genus Salamandrina) on the Apennine peninsula. J Zool 284:248–256

    Article  Google Scholar 

  • Hendrix R, Hauswaldt JS, Veith M, Steinfartz S (2010) Strong correlation between cross-amplification success and genetic distance across all members of “True Salamanders” (Amphibia: Salamandridae) revealed by Salamandra salamandra-specific microsatellite loci. Mol Ecol Res 10:1038–1047

    Article  CAS  Google Scholar 

  • Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106

    Article  PubMed  Google Scholar 

  • Mattoccia M, Marta S, Romano A, Sbordoni V (2011) Phylogeography of an Italian endemic salamander (genus Salamandrina): glacial refugia, postglacial expansions, and secondary contact. Biol J Linn Soc. doi:10.1111/j.1095-8312.2011.01747.x

  • Romano A, Mattoccia M, Marta S, Bogaerts S, Pasmans F, Sbordoni V (2009) Distribution, morphological characterization of the endemic Italian salamanders Salamandrina perspicillata (Savi, 1821), S. terdigitata (Bonnaterre, 1789) (Caudata: Salamandridae). Ital J Zool 76:422–432

    Article  CAS  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols in the series methods in molecular biology. Humana Press, Totowa, pp 365–386

    Google Scholar 

  • Steinfartz S, Vicario S, Arntzen JW, Caccone A (2007) A Bayesian approach on molecules and behavior: reconsidering phylogenetic and evolutionary patterns of the Salamandridae with emphasis on Triturus newts. J Exp Zool 308B:139–162

    Article  Google Scholar 

  • Zhang P, Papenfuss TJ, Wake MH, Qu L, Wake DB (2008) Phylogeny and biogeography of the family Salamandridae (Amphibia: Caudata) inferred from complete mitochondrial genomes. Mol Phylogenet Evol 49:586–597

    Article  PubMed  CAS  Google Scholar 

  • Zuffi MAL (1999) Salamandrina terdigitata (Lacépède, 1788)–Brillensalamander. In: Grossenbacher K, Thiesmeier B (eds) Handbuch der Reptilien und Amphibien Europas, band 4/1 Schwanzlurche (Urodela). Aula-Verlag, Wiesbaden, pp 229–246

    Google Scholar 

Download references

Acknowledgments

While at the University of Cologne, JSH was supported by a postdoctoral fellowship from the Max-Planck Institute for Evolutionary Biology in Plön. We would like to thank Diethard Tautz for his generous support and Jessica Füssel, Meike Kondermann, Eva Saxinger, and Gaby Keunecke for their help in the laboratory.

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Authors and Affiliations

  1. Unit of Evolutionary Biology, Institute of Zoology, TU Braunschweig, Mendelssohnstr. 4, 38106, Braunschweig, Germany

    J. Susanne Hauswaldt, Andy Polok & Claudio Angelini

  2. Institute of Genetics, University of Cologne, Zülpicher Strasse 47, 50674, Cologne, Germany

    J. Susanne Hauswaldt

  3. Department of Animal Behaviour, Unit Molecular Ecology and Behaviour, University of Bielefeld, Morgenbreede 45, 33615, Bielefeld, Germany

    Sebastian Steinfartz

Authors
  1. J. Susanne Hauswaldt
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  2. Andy Polok
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  3. Claudio Angelini
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  4. Sebastian Steinfartz
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Correspondence to J. Susanne Hauswaldt.

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Hauswaldt, J.S., Polok, A., Angelini, C. et al. First microsatellite loci for spectacled salamanders (Salamandrina perspicillata and S. terdigitata) endemic to the Apennine peninsula. Conservation Genet Resour 4, 399–402 (2012). https://doi.org/10.1007/s12686-011-9559-4

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  • DOI: https://doi.org/10.1007/s12686-011-9559-4

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