Abstract
As one of the major freshwater gastropod species, genetic diversity analysis of Bellamya was totally blank. Fifteen microsatellites loci were isolated and characterized from the freshwater snail Bellamya aeruginosa (Reeve, 1863). Most of the 15 loci had successful amplification and high polymorphic information content value (PIC), which ranged from 0.3174 to 0.8886 (average 0.7074). Allele number per locus ranged from 5 to 12 (average 8.4), the expected heterozygosity (H E ) and observed heterozygosity (H O ) varied from 0.6674 to 0.9073 and 0.0714 to 0.9130 with an average of 0.7985 and 0.6779, respectively. Six loci showed significant deviation from Hardy–Weinberg equilibrium (HWE) after Bonferroni correction (P < 0.0034) and no significant genotypic linkage disequilibrium was detected between most of locus pair, except for eight locus pairs. These fifteen polymorphic microsatellites loci would be precious for population genetics analysis and species identification of Bellamya.
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Acknowledgments
This work was financially supported by the National Natural Science Foundation of China (No. 31172410), the Specialized Research Fund for the Doctoral Program of Higher Education of China (No: 20090146110023) and the National Key Development Program (973) for Basic Research of China (No: 2009CB118706).
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Gu, Q., Xiong, B., Zhu, Y. et al. Isolation and characterization of polymorphic microsatellites loci in the freshwater snail Bellamya aeruginosa (Mollusca, Gastropoda). Conservation Genet Resour 4, 387–390 (2012). https://doi.org/10.1007/s12686-011-9556-7
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DOI: https://doi.org/10.1007/s12686-011-9556-7