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Characterization of 14 microsatellite loci developed for Dermacentor albipictus and cross-species amplification in D. andersoni and D. variabilis (Acari: Ixodidae)

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Abstract

Eleven polymorphic and three monomorphic microsatellite loci were developed for Dermacentor albipictus using three methods: (1) screening of a microsatellite enriched genomic DNA library, (2) in silico screening of EST libraries, and (3) adapting loci previously developed for D. variabilis (Say). An average of 4.91 alleles was detected per polymorphic locus. Average observed and expected heterozygosity among polymorphic loci was 0.518 and 0.623 respectively. No locus was significantly linked and one locus exhibited significant deviation from Hardy–Weinberg equilibrium. All loci amplified with varying success in D. andersoni and D. variabilis. These microsatellite loci will be useful for studying the species boundaries and genetic diversity of Dermacentor albipictus in North America.

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References

  • Alarcon-Chaidez FJ, Sun J, Wikel SK (2007) Transcriptome analysis of the salivary glands of Dermacentor andersoni stiles (Acari: Ixodidae). Insect Biochem Mol Biol 37(1):48–71

    Article  PubMed  CAS  Google Scholar 

  • Anderson JM, Sonenshine DE, Valenzuela JG (2008) Exploring the mialome of ticks: an annotated catalogue of midgut transcripts from the hard tick, Dermacentor variabilis (Acari: Ixodidae). BMC Genomics 9(1):552

    Article  PubMed  Google Scholar 

  • Aubry P, Geale DW (2011) A review of bovine anaplasmosis. Transbound Emerg Dis 58:1–30

    Article  PubMed  CAS  Google Scholar 

  • Beldade P, Rudd S, Gruber JD, Long AD (2006) A wing expressed sequence tag resource for Bicyclus anynana butterflies, an evo-devo model. BMC Genomics 7:130

    Article  PubMed  Google Scholar 

  • Dharmarajan G, Fike JA, Beasley JC, Rhodes OE (2009) Development and characterization of 12 polymorphic microsatellite loci in the American dog tick (Dermacentor variabilis). Mol Ecol Resour 9:131–133

    Article  PubMed  CAS  Google Scholar 

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Method Enzymol 395:202–222

    Article  CAS  Google Scholar 

  • Hamilton MB, Pincus EL, DiFiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. BioTechniques 27:500–507

    PubMed  CAS  Google Scholar 

  • Jaworski DC, Zou Z, Bowen CJ, Wasala NB, Madden R, Wang Y, Kocan KM, Jiang H, Dillwith JW (2010) Pyrosequencing and characterization of immune response genes from the American dog tick, Dermacentor variabilis (L.). Insect Mol Biol 19:617–630

    Google Scholar 

  • Jiang K, Shi Y, Zhang J, Xu N (2011) Microsatellite primers for vulnerable seagrass Halophila beccariia (Hydrocharitaceae). Am J Bot 98:155–157

    Article  Google Scholar 

  • Kelkar YD, Strubczewaki N, Hile SE, Chiaromonte F, Eckert KA, Makova KD (2010) What is a microsatellite: a computational and experimental definition based upon repeat mutational behaviour at A/T and GT/AC repeats. Genome Biol Evol 2:620–635

    Article  PubMed  Google Scholar 

  • Leo SST, Pybus MJ, Sperling FAH (2010) Deep mitochondrial DNA lineage divergences within Alberta populations of Dermacentor albipictus (Acari: Ixodidae) do not indicate distinct species. J Med Entomol 47:565–574

    Article  PubMed  CAS  Google Scholar 

  • Lumley LM, Sperling FAH (2011) Utility of microsatellites and mitochondrial DNA for species delimitation in the spruce budworm (Choristoneura fumiferana) species complex (Lepidoptera: Tortricidae). Mol Phylogenet Evol 58:232–243

    Article  PubMed  CAS  Google Scholar 

  • Park SDE (2001) Trypanotolerance in West African cattle and the population genetic effects of selection. Ph.D. thesis, University of Dublin

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386

    Google Scholar 

  • Samuel WM (2004) White as a ghost: winter ticks and moose. Federation of Alberta naturalists. Edmonton, Alberta

    Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

    Article  PubMed  CAS  Google Scholar 

  • van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

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Acknowledgments

We thank Simone Ciuti and his PhD supervisor Dr. M. Boyce for collecting D. albipictus specimens used in the validation panel, and Alberta Fish and Wildlife for the ticks from which gDNA were extracted; S. Drew, C. Sabin, and P. Pelli for providing D. variabilis specimens; J. Wilson for D. andersoni specimens. This study was funded by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada to Felix Sperling.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Alberta, CW 405 Biological Sciences Ctr., Edmonton, AB, T6G 2E9, Canada

    S. S. T. Leo, C. S. Davis & F. A. H. Sperling

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  1. S. S. T. Leo
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  2. C. S. Davis
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  3. F. A. H. Sperling
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Correspondence to S. S. T. Leo.

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Leo, S.S.T., Davis, C.S. & Sperling, F.A.H. Characterization of 14 microsatellite loci developed for Dermacentor albipictus and cross-species amplification in D. andersoni and D. variabilis (Acari: Ixodidae). Conservation Genet Resour 4, 379–382 (2012). https://doi.org/10.1007/s12686-011-9553-x

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  • DOI: https://doi.org/10.1007/s12686-011-9553-x

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