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Isolation and characterization of 39 novel polymorphic EST-SSR loci for the blood clam, Tegillarca granosa

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Abstract

We isolated and characterized 39 expressed sequence tag (EST)-derived simple sequence repeats (SSR) for the blood clam Tegillarca granosa. The number of alleles of the 39 EST-SSR markers varied from 2 to 8, with a mean of 4.0 alleles per locus. The number of observed, expected heterozygosity and polymorphic information content per locus varied from 0.200 to 1.000, from 0.549 to 0.841 and from 0.253 to 0.849, respectively. No significant deviations from Hardy–Weinberg equilibrium or linkage disequilibrium between pairs of loci were observed. These loci are firstly characterized and will be useful for investigation genetic population structure and molecule-assisted breeding for T. granosa.

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References

  • Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331

    PubMed  CAS  Google Scholar 

  • Jerry DR, Preston NP, Crocos PJ, Keys S, Meadows JRS, Li Y (2004) Parentage determination of Kuruma shrimp Penaeus (Marsupenaeus) japonicus using microsatellite markers (Bate). Aquaculture 235:237247

    Article  Google Scholar 

  • Liu YG, Chen SL, Li BF (2004) Assessing the genetic structure of three Japanese flounder (Paralichthys olivaceus) stocks by microsatellite markers. Aquaculture 243:103–111

    Article  Google Scholar 

  • Morgante M, Hanafey M, Powell W (2002) Microsatellites are preferentially associated with nonrepetitive DNA in plant genomes. Nat Genet 30:194–200

    Article  PubMed  CAS  Google Scholar 

  • Raymond M, Rousset F (1995) GenePop (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Simko I (2009) Development of EST-SSR markers for the study of population structure in lettuce (Lactuca sativa L.). J Hered 100(2):256–262

    Google Scholar 

  • Toth G, Gaspari Z, Jurka J (2000) Microsatellites in different eukaryotic genomes: surveys and analysis. Genome Res 10:967–981

    Article  PubMed  CAS  Google Scholar 

  • Wang RX, Li TW, Lu ZM, Su XR, Lin ZH, Chai XL (2005) Study on genetic variation by isozyme in different populations of bloody clam Tegillarca granosa. Oceanologia et Limnologia Sinica 36(3):227–234

    CAS  Google Scholar 

  • Wen YM, Gao RC (2009) Nutritive and officinal value of Tegillarca granosa. J Econ Anim 13(3):168–182

    CAS  Google Scholar 

Download references

Acknowledgments

This project was financially supported by Zhejiang Provincial Natural Science Foundation of China (Y3110003), National Shellfish Industry Technology System (CARS-48), Zhejiang Provincial Special Fund for Scientific Research Institution (2009F20010) and Wenzhou Science and Technology Program (S20080019).

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Authors and Affiliations

  1. Zhejiang Mariculture Research Institute, 6-1 Hetong Brodge, Wenzhou, 325000, China

    Bo Liu, Shuang-shuang Teng, Yan-Qing Shao, Xue-Liang Chai & Guo-Qiang Xiao

  2. Zhejiang Key Laboratory of Exploitation and Reservation of Coastat Bio-Resource, Wenzhou, 325000, China

    Bo Liu, Shuang-shuang Teng, Yan-Qing Shao, Xue-Liang Chai & Guo-Qiang Xiao

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  1. Bo Liu
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  2. Shuang-shuang Teng
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  3. Yan-Qing Shao
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  4. Xue-Liang Chai
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  5. Guo-Qiang Xiao
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Corresponding author

Correspondence to Xue-Liang Chai.

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Liu, B., Teng, Ss., Shao, YQ. et al. Isolation and characterization of 39 novel polymorphic EST-SSR loci for the blood clam, Tegillarca granosa . Conservation Genet Resour 4, 375–378 (2012). https://doi.org/10.1007/s12686-011-9552-y

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  • DOI: https://doi.org/10.1007/s12686-011-9552-y

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