Skip to main content
SpringerLink
Log in

Isolation and characterization of sixteen microsatellite loci of a blind cavefish, Triplophysa xiangxiensis

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

The blind loach, Triplophysa xiangxiensis, is an endemic fish in feihu cave of western Hunan Province of China. In this study, we describe the isolation and characterization of sixteen polymorphic microsatellite loci for T. xiangxiensis. The polymorphism of these markers was determined from 36 individuals. The number of alleles per locus ranged from 2 to 9. The observed and expected heterozygosity ranged from 0.3625 to 0.8918 and from 0.5386 to 0.8915, respectively. The data thus suggest their potential use as genetic markers for assessment of germplasm diversity and linkage analysis of T. xiangxiensis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Aljanabi SM, Martinez I (1997) Universal and salt-extracted of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res 25:4692–4693

    Article  PubMed  CAS  Google Scholar 

  • Chen XY, Cui GH, Yang JX (2004) A New Cave-dwelling fish species of genus Triplophysa (Balitoridae) from Guangxi. China Zoo Res 25(3):227–231

    CAS  Google Scholar 

  • He L, Wang XG, Cheng QC, Xiang JC (2006) Morphologic description on Triptophysa xiangxiensis. China Freshwater Fish 36(4):56–58

    Google Scholar 

  • Juan C, Guzik MT, Jaume D, Cooper SJB (2010) Evolution in caves: Darwin’s’ wrecks of ancient life’ in the molecular era. Mol Ecol 19:3865–3880

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386

    Google Scholar 

  • Vos P, Hogers R, Bleeker M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Yang GR, Yuang FX, Liao RM (1986) A new blind Cobitidae fish from the subterranean water in Xiangxi. China J Huazhong Agric Univ 5(3):219–223

    Google Scholar 

  • Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM (2000) POPGENE 32: microsoft windows-based freeware for population genetic analysis. 1.32. Molecular biology and biotechnology centre. University of Alberta, Edmonton

    Google Scholar 

  • Zane L, Bargelloni L, Patamello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Professor Guan Pin Yang from Ocean University of China for the help of technology.

Author information

Authors and Affiliations

  1. Fisheries College, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Yanhong Yao & Lai Ning Yu

  2. Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Wuhan, 430223, People’s Republic of China

    Yanhong Yao, Deng Qiang Wang, Li He & Lai Ning Yu

Authors
  1. Yanhong Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Deng Qiang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Li He
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lai Ning Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lai Ning Yu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yao, Y., Wang, D.Q., He, L. et al. Isolation and characterization of sixteen microsatellite loci of a blind cavefish, Triplophysa xiangxiensis . Conservation Genet Resour 4, 371–373 (2012). https://doi.org/10.1007/s12686-011-9551-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-011-9551-z

Keywords

Search

Navigation