Abstract
17 polymorphic microsatellite loci were screened and characterized in Octopus vulgaris using a microsatellite enriched genomic library. The number of alleles per polymorphic microsatellite locus ranged from 3 to 17, and the observed and expected heterozygosities varied from 0.154 to 1.000 and from 0.214 to 0.925, respectively. These microsatellite loci will be useful for further studies on the population structure and genetic variation of this species.
References
Cabranes C, Fernandez-Rueda P, Martinez JL (2008) Genetic structure of Octopus vulgaris around the Iberian Peninsula and Canary Islands as indicated by microsatellite DNA variation. J Mar Sci 65:12–16
Dieringer D, Schlötterer C (2003) Microsatellite analyser (MSA): a platform independent analysis tool for large microsatellite data sets. Mol Ecol Notes 3:167–169
Gardner MG, Cooper SJB, Bull CM, Grant WN (1999) Isolation of microsatellite loci from a social lizard, Egernia stokesii, using a modified enrichment procedure. J Hered 90:301–304
Greatorex EC, Jones CS, Murphy J, Key LN, Emery AM, Boyle PR (2000) Microsatellite markers for investigating population structure in Octopus vulgaris (Mollusca: Cephalopoda). Mol Ecol 9(5):641–642
Iglesias J, Sanchez FJ, Oteroj J, Moxica C (2000) Culture of octopus (Octopus vulgaris Cuvier): present knowledge, problems and perspectives. Ciheam Options Mediterr 47(11):313–322
Iglesias J, Oteroj J, Moxica C, Fuentes L, Sánchez FJ (2004) The completed life cycle of the octopus (Octopus vulgaris Cuvier) underculture conditions: paralarval rearing using Artemia and zoeae, and first data on juvenile growth up to 8 months of age. Aquacult Int 12(4/5):481–487
Li Q, Wan JM (2005) SSR hunter: development of a local searching software for SSR sites. Hereditas 27:808–810 (in Chinese with English abstract)
Murphy JM, Balguerıas E, Key LN, Boyle PR (2002) Microsatellite DNA markers discriminate between two Octopus vulgaris (Cephalopoda: Octopoda) fisheries along the Northwest African Coast. B Mar Sci 71:545–553
Oosthuizen A, Meesbah J, Shaw P (2004) Genetic analysis of the Octopus vulgaris population the coast of South Africa. S Afr J Sci 100:603–607
Quinteiro J, Baibai T, Oukhattar L, Soukri A, Seixas P, Rey-Mendez M (2011) Multiple paternity in the common octopus Octopus vulgaris (Cuvier, 1797), as revealed by microsatellite DNA analysis. Molluscan Res 31(1):15–20
Roper CFE, Sweeney MJ, Nauen CE (1984) FAO species catalogue. Vol. 3. Cephalopods of the world. An annotated and illustrated catalogue of species of interest to fisheries. FAO Fish Synop 125(3):211–212
Rousset F (2008) GENEPOP’O007: a complete reimplementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106
Vazpires P, Seixas P, Barbos A (2004) Aquaculture potential of the common octopus (Octopus vulgaris Cuvier, 1797): a review. Aquaculture 238(1/4):221–228
Warnke K, Soller R, Blohm D, Saint-Paul U (2004) A new look at geographic and phylogenetic relationships within the species group surrounding Octopus vulgaris (Mollusca, Cephalopoda): indications of very wide distribution from mitochondrial DNA sequences. J Zool Syst Evol Res 42(4):306–312
Zheng XD, Liu ZS, Zhao N, An WT, Yang SJ, Lin XZ (2011) Embryonic development and paralarval growth of Octopus vulgaris. Oceanlogia et Limnologia Sini 42(2):318–323
Acknowledgments
We thank Z. S. Liu (Fisheries College, Ocean University of China) for collecting samples and Prof. C. C. Lu for proofreading. The study was supported by the grants from Shandong Outstanding Young Scientist Research Award Fund (2008BS06008) and the National High Technology Research and Development Program of China (863 Program) (2007AA09Z433).
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Zuo, Z., Zheng, X., Liu, C. et al. Development and characterization of 17 polymorphic microsatellite loci in Octopus vulgaris Cuvier, 1797. Conservation Genet Resour 4, 367–369 (2012). https://doi.org/10.1007/s12686-011-9550-0
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DOI: https://doi.org/10.1007/s12686-011-9550-0