Abstract
The Asian swamp eel Monopterus albus is distributed in rice fields, rivers and ponds. Due to large scale use of pesticides in rice fields and destruction of natural habitats, the wild populations are decreasing very quickly. To facilitate studies on genetic diversity, we isolated 16 polymorphic novel microsatellites and characterized them using 48 individuals from two natural populations from two locations. The average allele number of the 16 microsatellites was 10.1/locus, ranging from 2 to 20. The expected heterozygosity was 0.79 whereas the observed heterozygosity was 0.65. In both populations, all markers conformed to HWE and showed no significant linkage between pairwise markers. The genetic differentiation between the two populations was significant (F ST = 0.24, P < 0.01). In addition, we developed two sets of multiplex PCR to fast genotype 11 loci. This set of microsatellites provides a tool for assessing genetic diversity and population structure of this species.
This is a preview of subscription content, log in via an institution to check access.
References
Fischer D, Bachmann K (1998) Microsatellite enrichment in organisms with large genomes (Allium cepa L.). Biotechniques 24:796–802
Goudet J (1995) FSATA (vers.1.2): a computer program to calculate F-statistics. J Hered 85:485–486
Guan R, Zhou L, Cui G, Feng X (1996) Studies on the artificial propagation of Monopterus albus (Zuiew). Aqua Res 27:587–596
Lewis PO, Zaykin D (2000) Genetic data analysis. Available at http://hydrodictyon.eeb.uconn.edu/people/plewis/software.php
Liem KF (1963) Sex reversal as a natural process in the synbranchiform fish Monopterus albus. Copeia 2:303–312
Liu F, Xia JH, Bai ZY, Fu JJ, Li JL, Yue GH (2009) High genetic diversity and substantial population differentiation in grass carp (Ctenopharyngodon idella) revealed by microsatellite analysis. Aquaculture 297:51–56
Nelson JS (ed) (2006) Fishes of the world, 4th edn. Wiley, New York
Spruell P, Rieman B, Knudsen K, Utter F, Allendorf F (1999) Genetic population structure within streams: microsatellite analysis of bull trout populations. Ecol Freshwater Fish 8:114–121
Tao YX, Lin HR, Van Der Kraak G, Peter RE (1993) Hormonal induction of precocious sex reversal in the ricefield eel, Monopterus albus. Aquaculture 118:131–140
Yue GH, Orban L (2001) Rapid isolation of DNA from fresh and preserved fish scales for polymerase chain reaction. Mar Biotechnol 3:199–204
Yue GH, Beeckmann P, Bartenschlager H, Moser G, Geldermann H (1999) Rapid and precise genotyping of porcine microsatellites. Electrophoresis 20:3358–3363
Yue GH, Chen F, Orban L (2000) Rapid isolation and characterization of microsatellites from the genome of Asian arowana (Scleropages formosus, Osteoglossidae, Pisces). Mol Ecol 9:1007–1009
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lei, L., Feng, L., Jian, T.R. et al. Characterization and multiplex genotyping of novel microsatellites from Asian swamp eel, Monopterus albus . Conservation Genet Resour 4, 363–365 (2012). https://doi.org/10.1007/s12686-011-9549-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-011-9549-6