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Twenty-four additional microsatellite markers derived from expressed sequence tags of the endangered tropical tree Shorea leprosula (Dipterocarpaceae)

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Abstract

We developed microsatellite markers from newly isolated expressed sequence tags of an endangered tropical tree species, Shorea leprosula (Dipterocarpaceae). Twenty-four loci exhibited clear, polymorphic amplification patterns among 52 individuals from a population in Indonesia, with two to 16 alleles per locus. No locus showed significant departure from Hardy–Weinberg equilibrium, and no significant genotypic disequilibrium was detected for any pair of loci after sequential Bonferroni correction.

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Acknowledgments

The authors thank Y. Taguchi and M. Koshiba for laboratory work and members of the Forest Research Institute Malaysia for their assistance during sample collection. This study was supported by a Grant-in-Aid for Scientific Research (No. 18255010) provided by the Ministry of Education, Culture, Sports, Science and Technology of Japan and the Global Environment Research Program supported by the Japanese Ministry of Environment, Grant No. E-091.

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Author notes

  1. Masato Ohtani

    Present address: Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Hitachi, Ibaraki, 319-1301, Japan

Authors and Affiliations

  1. Department of Forest Genetics, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, 305-8687, Japan

    Masato Ohtani, Saneyoshi Ueno & Yoshihiko Tsumura

  2. Forestry Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, 305-8686, Japan

    Naoki Tani

  3. Forest Research Institute Malaysia, 52109, Kepong, Selangor, Malaysia

    Leong Soon Lee

Authors
  1. Masato Ohtani
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  2. Saneyoshi Ueno
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  3. Naoki Tani
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  4. Leong Soon Lee
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  5. Yoshihiko Tsumura
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Corresponding author

Correspondence to Yoshihiko Tsumura.

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Ohtani, M., Ueno, S., Tani, N. et al. Twenty-four additional microsatellite markers derived from expressed sequence tags of the endangered tropical tree Shorea leprosula (Dipterocarpaceae). Conservation Genet Resour 4, 351–354 (2012). https://doi.org/10.1007/s12686-011-9546-9

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  • DOI: https://doi.org/10.1007/s12686-011-9546-9

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