Abstract
Wyoming toad (Bufo baxteri) is an endangered amphibian native to the Laramie Basin, Wyoming, USA. A captive breeding program propagates B. baxteri, and the monitoring of genetic diversity in captive stock can assist in guiding restoration and recovery efforts. We developed eight species-specific polymorphic loci from an enriched microsatellite library. For 281 samples, B. baxteri exhibited 2–11 alleles per locus, and observed and expected heterozygosities per locus ranged from 0.411 to 0.943 and from 0.400 to 0.691, respectively. Four loci deviated significantly from Hardy–Weinberg equilibrium. The eight microsatellite markers may be useful for conservation, population, and quantitative genetics for B. baxteri and closely related species.
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Acknowledgments
Funding and administration for this research were provided by Jan McKee, US Fish and Wildlife Service. We thank Lee Bender, Dave Paddock, and Paul Larson at Saratoga NFH for sample collection, Manuel Ulibarri and staff at the Dexter NFHTC, and Wyoming Toad Recovery Team. Additionally, we thank Drs. Michele K. Nishiguchi and Wiebke J. Boeing at NMSU for helpful insights. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of the US Fish and Wildlife Service.
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Martin, R.M., Keeler-Foster, C.L., Boykin, K.G. et al. Isolation and characterization of eight novel microsatellite loci in endangered Wyoming toad, Bufo baxteri . Conservation Genet Resour 4, 347–349 (2012). https://doi.org/10.1007/s12686-011-9544-y
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DOI: https://doi.org/10.1007/s12686-011-9544-y