Conservation Genetics Resources

, Volume 4, Issue 2, pp 343–345 | Cite as

Novel tetra- and pentanucleotide microsatellite markers allow for multiplexed genotyping of Sulawesi tarsiers (Tarsius spp.)

  • Stefan Merker
  • Désirée Boucsein
  • Barbara Feldmeyer
  • Dyah Perwitasari-Farajallah
  • Bruno Streit
Technical Note
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Abstract

Based on 454 sequencing, we characterized 10 polymorphic tetra- and pentanucleotide microsatellites for population studies of Sulawesi tarsiers. We identified 2516 repeat regions and designed PCR primer pairs for 60 tetra-, penta- and hexanucleotide repeat loci. Of 16 markers tested with dye-labelled primers, 11 proved to be polymorphic and 10 were readily amplified in multiplex PCR reactions. We tested 54 individuals of three species (T. lariang, T. dentatus, T. wallacei) and 10 putative hybrids for allelic diversity. All loci are genetically unlinked, one locus was found to deviate from Hardy–Weinberg equilibrium in one species. The novel marker system will aid studies of tarsier landscape genetics in the biodiversity hotspot Sulawesi.

Keywords

454 Sequencing Microsatellites Primates Sulawesi Tarsius 

References

  1. Driller C, Perwitasari-Farajallah D, Zischler H, Merker S (2009) The social system of Lariang tarsiers (Tarsius lariang) as revealed by genetic analyses. Int J Primatol 30:267–281CrossRefGoogle Scholar
  2. Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567PubMedCrossRefGoogle Scholar
  3. IUCN (2011) IUCN red list of threatened species. Version 2011.1. Available at www.iucnredlist.org. Downloaded on 16 September 2011
  4. Merker S, Driller C, Perwitasari-Farajallah D, Zahner R, Zischler H (2007) Isolation and characterization of 12 microsatellite loci for population studies of Sulawesi tarsiers (Tarsius spp.). Mol Ecol Notes 7:1216–1218CrossRefGoogle Scholar
  5. Merker S, Driller C, Neri-Arboleda I, Zischler H (2008) Polymorphic microsatellite markers for Philippine tarsiers (Tarsius syrichta). Conserv Genet 9:1349–1351CrossRefGoogle Scholar
  6. Merker S, Driller C, Perwitasari-Farajallah D, Pamungkas J, Zischler H (2009) Elucidating geological and biological processes underlying the diversification of Sulawesi tarsiers. Proc Natl Acad Sci USA 106:8459–8464PubMedCrossRefGoogle Scholar
  7. Merker S, Driller C, Dahruddin H, Wirdateti, Sinaga W, Perwitasari-Farajallah D, Shekelle M (2010) Tarsius wallacei: a new tarsier species from Central Sulawesi occupies a discontinuous range. Int J Primatol 31:1107–1122CrossRefGoogle Scholar
  8. Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295CrossRefGoogle Scholar
  9. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Stefan Merker
    • 1
  • Désirée Boucsein
    • 1
  • Barbara Feldmeyer
    • 2
  • Dyah Perwitasari-Farajallah
    • 3
    • 4
  • Bruno Streit
    • 1
  1. 1.Evolutionary Ecology GroupGoethe University FrankfurtFrankfurt am MainGermany
  2. 2.Molecular Ecology GroupForschungszentrum Biodiversität und KlimaFrankfurt am MainGermany
  3. 3.Primate Research CenterBogor Agricultural UniversityBogorIndonesia
  4. 4.Department of BiologyBogor Agricultural UniversityBogorIndonesia

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