Abstract
Ten microsatellite loci were isolated and characterized from the spiny-rat (Clyomy bishopi), a medium-size Neotropical rodent that inhabits some of the last remaining savannas from São Paulo state, Brazil. Between 5 and 17 alleles were detected per locus, with expected heterozygosity ranging from 0.605 to 0.921. All but one locus were found to be in Hardy–Weinberg equilibrium, and no linkage disequilibrium was detected among pairs of loci. These microsatellites should provide useful markers in genetic studies including parentage analyses and determining genetic and social structure of populations.
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Acknowledgments
The authors thank Instituto Florestal de São Paulo (IF process no. 260108-014.277/2009) and Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio process no. 21075-1) for collecting permits; to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for fellowship to ACRA and to Prof Pedro M. Galetti-Júnior for allowing the use of his laboratory facilities at UFSCar.
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Arantes, A.C.R., Trovati, R.G., Peripato, A.C. et al. Isolation and characterization of polymorphic microsatellite loci for the spiny-rat Clyomys bishopi (Rodentia: Echimyidae). Conservation Genet Resour 4, 335–337 (2012). https://doi.org/10.1007/s12686-011-9541-1
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DOI: https://doi.org/10.1007/s12686-011-9541-1