Isolation via 454 sequencing, and characterisation of microsatellites for Drymodes brunneopygia, southern scrub-robin (Aves: Petroicidae): a species at risk due to substantial habitat loss and climate change
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We isolated 10 new polymorphic microsatellite markers from Drymodes brunneopygia, a ground-dwelling bird species distributed in semi-arid mallee habitat across southern Australia. Initially 454 shotgun sequencing was used to identify 51 loci for which primers were designed. We trialled all 51 loci in the target species and 29 (57%) amplified a polymorphic product of expected size. Subsequently 18 of these loci were screened for variation in 38 individuals from Billiatt Conservation Park in South Australia. All loci were polymorphic; however, only 10 loci could be scored reliably. For those 10 loci, observed heterozygosity ranged from 0.105 to 0.842 (mean 0.634) and the number of alleles per locus ranged from 2 to 10 (average of 6.7). These loci will be useful for characterising genetic variation and metapopulation structure in Drymodes brunneopygia, a species threatened by agriculture and climate change.
KeywordsDrymodes brunneopygia Southern scrub-robin Microsatellites 454 GS-FLX Shotgun sequencing
We acknowledge the assistance of Kathy Saint (University of Adelaide) and Alison Fitch (Flinders University). This project was funded by CSIRO, Department of Environment and Natural Resources in South Australia, Sir Mark Mitchell Research Foundation, Birds Australia and Australian Geographic Society.
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