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Development of novel microsatellite markers for the invasive Northern Pacific seastar, Asterias amurensis

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Abstract

The Northern Pacific seastar, Asterias amurensis, is a benthic marine predator, which has recently established several invasive populations in Australian waters. To investigate population structure, diversity and patterns of connectivity, we isolated and characterised 27 microsatellite loci and tested their polymorphism based on 46 individuals from two invasive populations. The mean allelic richness was 4.33; observed heterozygosity was 0.42, while the percentage of polymorphic loci was 92.6%. The polymorphic markers will prove useful in the assessment of population genetic parameters, in both invasive and native A. amurensis populations.

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References

  • Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8(1):92–94. doi:10.1111/j.1471-8286.2007.01884.x

    Article  PubMed  CAS  Google Scholar 

  • Gardner MG, Fitch AJ, Bertozzi T, Lowe AJ (2011) Rise of the machines—recommendations for ecologists when using next generation sequencing for microsatellite development. Mol Ecol Resour. doi:10.1111/j.1755-0998.2011.03037.x

  • Goggin LC (1998) Technical Report 15: Proceedings of a meeting on the biology and management of the introduced seastar, Asterias amurensis, in Australian waters. CSIRO Marine Research

  • Grosholz E (2002) Ecological and evolutionary consequences of coastal invasions. Trends Ecol Evol 17(1):22–27

    Article  Google Scholar 

  • Lee CE (2002) Evolutionary genetics of invasive species. Trends Ecol Evol 17(8):386–391

    Article  Google Scholar 

  • Molnar JL, Gamboa RL, Revenga C, Spalding MD (2008) Assessing the global threat of invasive species to marine biodiversity. Front Ecol Environ 6(9):485–492. doi:10.1890/070064

    Article  Google Scholar 

  • Parry G, Heislers S, Werner G (2004) Changes in distribution and abundance of Asterias amurensis in Port Phillip Bay 1999–2003. Department of Primary Industries

  • Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6(1):288–295. doi:10.1111/j.1471-8286.2005.01155.x

    Article  Google Scholar 

  • Raymond M, Rousset F (1995) Genepop (Version-1.2)—population-genetics software for exact tests and ecumenicism. J Hered 86(3):248–249

    Google Scholar 

  • Reusch TBH, Bolte S, Sparwel M, Moss AG, Javidpour J (2010) Microsatellites reveal origin and genetic diversity of Eurasian invasions by one of the world’s most notorious marine invader, Mnemiopsis leidyi (Ctenophora). Mol Ecol 19(13):2690–2699. doi:10.1111/j.1365-294X.2010.04701.x

    Article  PubMed  CAS  Google Scholar 

  • Ross DJ, Johnson CR, Hewitt CL (2003) Assessing the ecological impacts of an introduced seastar: the importance of multiple methods. Biol Invasions 5:3–21

    Article  Google Scholar 

  • Ross DJ, Johnson CR, Hewitt CL (2006) Abundance of the introduced seastar, Asterias amurensis, and spatial variability in soft sediment assemblages in SE Tasmania: clear correlations but complex interpretation. Estuar Coast Shelf Sci 67(4):695–707. doi:10.1016/j.ecss.2005.11.038

    Article  Google Scholar 

  • Sakai AK, Allendorf FW, Holt JS, Lodge DM, Molofsky J, With KA, Baughman S, Cabin RJ, Cohen JE, Ellstrand NC, McCauley DE, O’Neil P, Parker IM, Thompson JN, Weller SG (2001) The population biology of invasive species. Annu Rev Ecol Syst 32:305–332

    Article  Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4(3):535–538. doi:10.1111/j.1471-8286.2004.00684.x

    Article  Google Scholar 

  • Vitousek PM, DAntonio CM, Loope LL, Rejmanek M, Westbrooks R (1997) Introduced species: a significant component of human-caused global change. New Zeal J Ecol 21(1):1–16

    Google Scholar 

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Acknowledgments

We would like to thank J. Ross and J. Keane for help with obtaining samples from Tasmania and A. Miller for technical assistance. The Holsworth Wildlife Research Endowment, awarded to MFR, and a Central Research Grant Scheme grant, awarded to CDHS, supported research.

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Correspondence to Mark F. Richardson.

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Richardson, M.F., Stanley, A.M. & Sherman, C.D.H. Development of novel microsatellite markers for the invasive Northern Pacific seastar, Asterias amurensis . Conservation Genet Resour 4, 327–330 (2012). https://doi.org/10.1007/s12686-011-9539-8

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