Abstract
The Northern Pacific seastar, Asterias amurensis, is a benthic marine predator, which has recently established several invasive populations in Australian waters. To investigate population structure, diversity and patterns of connectivity, we isolated and characterised 27 microsatellite loci and tested their polymorphism based on 46 individuals from two invasive populations. The mean allelic richness was 4.33; observed heterozygosity was 0.42, while the percentage of polymorphic loci was 92.6%. The polymorphic markers will prove useful in the assessment of population genetic parameters, in both invasive and native A. amurensis populations.
References
Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8(1):92–94. doi:10.1111/j.1471-8286.2007.01884.x
Gardner MG, Fitch AJ, Bertozzi T, Lowe AJ (2011) Rise of the machines—recommendations for ecologists when using next generation sequencing for microsatellite development. Mol Ecol Resour. doi:10.1111/j.1755-0998.2011.03037.x
Goggin LC (1998) Technical Report 15: Proceedings of a meeting on the biology and management of the introduced seastar, Asterias amurensis, in Australian waters. CSIRO Marine Research
Grosholz E (2002) Ecological and evolutionary consequences of coastal invasions. Trends Ecol Evol 17(1):22–27
Lee CE (2002) Evolutionary genetics of invasive species. Trends Ecol Evol 17(8):386–391
Molnar JL, Gamboa RL, Revenga C, Spalding MD (2008) Assessing the global threat of invasive species to marine biodiversity. Front Ecol Environ 6(9):485–492. doi:10.1890/070064
Parry G, Heislers S, Werner G (2004) Changes in distribution and abundance of Asterias amurensis in Port Phillip Bay 1999–2003. Department of Primary Industries
Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6(1):288–295. doi:10.1111/j.1471-8286.2005.01155.x
Raymond M, Rousset F (1995) Genepop (Version-1.2)—population-genetics software for exact tests and ecumenicism. J Hered 86(3):248–249
Reusch TBH, Bolte S, Sparwel M, Moss AG, Javidpour J (2010) Microsatellites reveal origin and genetic diversity of Eurasian invasions by one of the world’s most notorious marine invader, Mnemiopsis leidyi (Ctenophora). Mol Ecol 19(13):2690–2699. doi:10.1111/j.1365-294X.2010.04701.x
Ross DJ, Johnson CR, Hewitt CL (2003) Assessing the ecological impacts of an introduced seastar: the importance of multiple methods. Biol Invasions 5:3–21
Ross DJ, Johnson CR, Hewitt CL (2006) Abundance of the introduced seastar, Asterias amurensis, and spatial variability in soft sediment assemblages in SE Tasmania: clear correlations but complex interpretation. Estuar Coast Shelf Sci 67(4):695–707. doi:10.1016/j.ecss.2005.11.038
Sakai AK, Allendorf FW, Holt JS, Lodge DM, Molofsky J, With KA, Baughman S, Cabin RJ, Cohen JE, Ellstrand NC, McCauley DE, O’Neil P, Parker IM, Thompson JN, Weller SG (2001) The population biology of invasive species. Annu Rev Ecol Syst 32:305–332
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4(3):535–538. doi:10.1111/j.1471-8286.2004.00684.x
Vitousek PM, DAntonio CM, Loope LL, Rejmanek M, Westbrooks R (1997) Introduced species: a significant component of human-caused global change. New Zeal J Ecol 21(1):1–16
Acknowledgments
We would like to thank J. Ross and J. Keane for help with obtaining samples from Tasmania and A. Miller for technical assistance. The Holsworth Wildlife Research Endowment, awarded to MFR, and a Central Research Grant Scheme grant, awarded to CDHS, supported research.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Richardson, M.F., Stanley, A.M. & Sherman, C.D.H. Development of novel microsatellite markers for the invasive Northern Pacific seastar, Asterias amurensis . Conservation Genet Resour 4, 327–330 (2012). https://doi.org/10.1007/s12686-011-9539-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-011-9539-8