Abstract
Rhinogobio cylindricus is an endemic freshwater fish species with relevant commercial importance in the Yangtze River, China. Nineteen polymorphic microsatellite loci were developed by fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. The number of alleles, observed and expected heterozygosity ranged from 2 to 6, from 0.3333 to 1.000 and from 0.2943 to 0.7695, respectively. Ten loci were highly informative, nine were moderately informative. Six loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. Two groups of couple loci exhibited significant linkage disequilibrium. These polymorphic microsatellite loci will provide useful genetic tools for population study and conservation genetics of R. cylindricus.
This is a preview of subscription content, log in via an institution to check access.
References
Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Human Genet 32:314–331
Ding R (1994) Sichuan fish volunteers. Sichuan Sci Technol, Chendu, pp 282–284 (in Chinese with English abstract)
Li Q, Wan J (2005) SSRHunter: development of a local searching software for SSR sites. Hereditas 5:808–810 (in Chinese with English abstract)
Liu H, Xiong F, Yang D, Zhang F, Yu L (2011) Mitochondrial cytochrome b gene sequence diversity in wild and cultured populations of Anabarilius grahami. Agri Univ 30:94–98 (in Chinese with English abstract)
Ma H, He X (2004) Age and growth of Rhinogobio cylindricus in the mainstream of Yangtze River. Chinese J Zool 39:55–59 (in Chinese with English abstract)
Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Yeh FC, Yang RC, Boyle T (1999) POPGENE version 1.3.1. microsoft window-bases freeware for population genetic analysis. Available at www.ualberta.ca/~fyeh/. University of Alberta and the Centre for International Forestry Research
Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16
Acknowledgments
We thank Cunyao Li and Geng Chen for the technical assistance. This work was supported by the National Science Foundation (51109091), Special Fund for Agro-scientific Research in the Public Interest of China (200903048) and the Fund of Wuhan Bureau of Education (2010060).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liu, H.Y., Xiong, F., Duan, X.B. et al. A first set of polymorphic microsatellite loci isolated from Rhinogobio cylindricus . Conservation Genet Resour 4, 307–310 (2012). https://doi.org/10.1007/s12686-011-9531-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-011-9531-3