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Development and characterization of sixteen microsatellite loci for Geosmithia morbida, the causal agent of thousand canker disease in black walnut (Juglans nigra)

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Abstract

Sixteen polymorphic microsatellite loci were identified from the fungal pathogen Geosmithia morbida. Loci were characterized for 13 different isolates collected in 2010 from symptomatic black walnut trees in Tennessee. A total of 77 loci were tested and 16 of those were optimized, screened and selected for diversity studies of G. morbida. Number of alleles per locus ranged from 3 to 8. These microsatellite loci will be useful for rapid disease diagnostic, population genetic analyses on a global scale as well as further epidemiological studies of G. morbida.

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Acknowledgments

The authors thank the United States Department of Agriculture (Grant number 58-6404-7-213) for financial support.

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Correspondence to Denita Hadziabdic.

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Hadziabdic, D., Wadl, P.A., Vito, L.M. et al. Development and characterization of sixteen microsatellite loci for Geosmithia morbida, the causal agent of thousand canker disease in black walnut (Juglans nigra). Conservation Genet Resour 4, 287–289 (2012). https://doi.org/10.1007/s12686-011-9526-0

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