Isolation and characterization of 21 microsatellite loci for the federally threatened yellowfin madtom (Noturus flavipinnis) with cross species amplification in N. baileyi

Abstract

We isolated 21 microsatellite loci from Noturus flavipinnis, a federally threatened freshwater fish. Nine loci were polymorphic with an average of 1.48 alleles and an average observed and expected heterozygosity of 0.092 and 0.096, respectively. All loci conformed to Hardy–Weinberg expectations, and all pairs of loci showed no signs of genotypic disequilibrium. Three of these loci were found to cross amplify in N. baileyi. These markers should prove useful in the conservation efforts and genetic monitoring of these rare fish species.

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Acknowledgments

We thank P. Rakes and J. R. Shute of Conservation Fisheries Inc. for the collection of madtom samples and Genetic Identification Services for the construction and sequencing of enriched microsatellite libraries. Use of trade names throughout the manuscript does not constitute endorsement by the United States government.

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Correspondence to A. S. Williams.

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Williams, A.S., Moyer, G.R. Isolation and characterization of 21 microsatellite loci for the federally threatened yellowfin madtom (Noturus flavipinnis) with cross species amplification in N. baileyi . Conservation Genet Resour 4, 221–223 (2012). https://doi.org/10.1007/s12686-011-9511-7

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Keywords

  • Federally endangered species
  • Yellowfin madtom
  • Cross-species amplification
  • Microsatellites