Characterisation of polymorphic microsatellite loci in Hephaestus fuliginosus and cross-amplification in closely related Hephaestus tulliensis

Abstract

Eleven polymorphic microsatellite loci were isolated and characterised from sooty grunter (Hephaestus fuliginosus) and Tully grunter (H. tulliensis) from Northern Australia. Each primer pair amplified good quality and polymorphic products. The versatilities of these markers were tested using different H. fuliginosus populations and cross-amplified to a closely related species, H. tulliensis, found within the northern Australian wet tropics. The number of alleles ranged from 5 to 20 per locus and expected heterozygosities ranged from 0.043 to 0.926. All loci conformed to Hardy–Weinberg expectations, with the exception of 2Hf44 and 2Hf53 for Daly R. and Mitchell R. populations of H. fuliginosus, respectively. No evidence of linkage disequilibrium was detected between any pair of loci. The markers reported here would be very useful for population genetic studies, evolution and conservation of these species.

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Acknowledgments

This work was supported by Tropical Rivers and Coastal Knowledge Commonwealth Environmental Research Facility (TRaCK) that receives major funding through Australian Government’s Commonwealth Environment Research facilities initiative, Australian Government’s Raising National Water Standards Program, Land and Water Australia, Fisheries Research and Development Corporation, and Queensland Government’s Smart State Innovation Fund. We thank Courtenay Mills and Andrew Bentley for providing the samples. Brian Wade Jamandre is supported by the Endeavour Research Awards of the Australian Government’s Department of Education, Employment and Work Relations.

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Correspondence to Brian Wade Jamandre.

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Jamandre, B.W., Real, K. & Hughes, J. Characterisation of polymorphic microsatellite loci in Hephaestus fuliginosus and cross-amplification in closely related Hephaestus tulliensis . Conservation Genet Resour 4, 213–216 (2012). https://doi.org/10.1007/s12686-011-9509-1

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Keywords

  • Population genetic analysis
  • Northern Australia
  • Freshwater fish
  • Grunters