Abstract
We developed and characterized 20 microsatellite primer loci for the northern shrimp Pandalus borealis. All 20 loci were polymorphic with number of alleles ranging from 3 to 36 and with observed heterozygosity between 0.04 and 0.93. In addition, we tested the utility of these markers in three related shrimp species, P. montagui, Atlantopandalus propinqvus and Dichelopandalus bonnieri. These new markers will prove useful in the identification of stock structure and hence, assessment of the commercially important species P. borealis.
This is a preview of subscription content, log in via an institution to check access.
References
Drengstig A, Fevolden SE, Galand PE, Aschan MM (2000) Population structure of the deeps-sea shrimp (Pandalus borealis) in the north-east Atlantic based on allozyme variation. Aquat Living Res 13:121–128
Excoffier L, Lischer HEL (2010) Arlequin suite ver. 3.5: a new series of programs to perform population genetic analyses under Linux and Windows. Mol Ecol Res 10:564–567
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Meth Enzymol 395:202–222
Grant WS, Clark AM, Bowen BW (1998) Why restriction fragment length polymorphism analysis of mitochondrial DNA failed to resolve sardine (Sardinops) biogeography: insights from mitochondrial DNA cytochrome b sequences. Can J Fish Aquat Sci 55:2539–2547
Jónsdóttir ÓDB, Imsland AK, Nævdal G (1998) Population genetic studies of northern shrimp, Pandalus borealis, in Icelandic waters and the Denmark Strait. Can J Aquat Sci 55:770–780
Kartavtsev YP, Berenboim BI, Zgurovsky KI (1991) Population genetic differentiation of the pink shrimp Pandalus borealis Krøyer, 1838, from the Barents and Berings Seas. J Shellfish Res 10:333–339
Martinez I, Aschan M, Sjerdal T, Aljanabi SM (2006) The genetic structure of Pandalus borealis in the Northeast Atlantic determined by RAPD analysis. ICES J Mar Sci 63:840–850
Meredith EP, May B (2002) Microsatellite loci in Lahontan tui chub, Gila bicolor obese, and their utilization in other chub species. Mol Ecol Notes 2:156–158
Rasmussen T, Thollesson M, Nilssen EM (1993) Preliminary investigations on the population genetic differentiation of the deep water prawn, Pandalus borealis KRØYER 1838, from Northern Norway and the Barents Sea. ICES Document CM 1993/K:11
Ruzzante D, Mariani S, Bekkevold D, André C, Mosegaard H, Clausen L, Dahlgren T, Hutchinson W, Hatfield E, Torstensen E, Brigham J, Simmonds J, Laikre L, Larsson L, Stet R, Ryman N, Carvalho G (2006) Biocomplexity in a highly migratory marine pelagic fish. Proc Royal Soc Lon Ser B 273:1459–1464
Schindler DE, Hilborn R, Chasco B, Boatright CP, Quinn TP, Rogers LA, Webster MS (2010) Population diversity and the portfolio effect in an exploited species. Nature 465:609–613
Schwartz RS, May B (2004) Characterization of microsatellite loci in Sacramento perch (Archoplites interruptus). Mol Ecol Notes 4:694–697
Acknowledgments
We thank Mats Ulmestrand (Swedish National Board of Fisheries), P. E. Jorde, Endre Willassen (MAR-ECO, http://www.mar-eco.no, a field project under the Census of Marine Life programme) and local fishermen for good advice and for providing samples. We also thank Benno Jönsson, Kate Enersen and Hanne Sannæs for technical assistance. Funding was provided by the Norwegian Research Council project “POPBOREALIS”, European Regional Development Fund (ERDF), Nordic Working group for Fisheries Cooperation (AG-Fisk)/Nordic Council of Ministers and the EU Interreg IVA project “Sustainable fishery of Pandalus borealis”.
Author information
Authors and Affiliations
Corresponding author
Additional information
Ricardo T. Pereyra and Jon-Ivar Westgaard equally contributed to this work.
Rights and permissions
About this article
Cite this article
Pereyra, R.T., Westgaard, JI., Dahl, M. et al. Isolation and characterization of nuclear microsatellite loci in the northern shrimp, Pandalus borealis . Conservation Genet Resour 4, 109–112 (2012). https://doi.org/10.1007/s12686-011-9486-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-011-9486-4