Abstract
We describe the characterization of ten polymorphic microsatellite loci from the Liolaemus fitzingerii species complex with cross-amplification for nine of the 10 loci in L. chehuachekenk. The number of alleles within L. fitzingerii ranged from 7 to 24 and the observed heterozygosity ranged from 0.311 to 0.956 for 45 individuals. Nine of the ten loci conformed to Hardy–Weinberg equilibrium and we did not detect linkage disequilibrium between loci in L. fitzingerii. Cross-species amplification in L. chehuachekenk was successful for nine of the ten loci with polymorphisms detected for each successfully amplified locus. These microsatellite loci are the first developed for the Eulaemus clade of the genus, and will aid in both ongoing and future studies focusing on gene flow, historical divergence and population structure within the Liolaemus fitzingerii species complex.
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References
Avila LJ, Morando M, Sites JW Jr (2006) Cogneric phylogeography: hypothesizing species limits and evolutionary processes in Patagonian lizards fo the Liolaemus boulengeri group (Squamata: Liolaemini). Biol J Linn Soc 89:249–275
Cei JM (1986) Reptiles del centro, centro-oeste y sur de la Argentina. Museo Regionale di Scienze Naturali, Torino, Monografie 4, pp 1–527
Cei JM (1993) Reptiles del noroeste, nordeste y este de la Argentina. Museo Regionale di Scienze Naturali, Torino, Monografie 14, pp 1–949
Etheridge R (1993) Lizards of the Liolaemus darwinii complex (Squamata: Iguania: Tropiduridae) in Northern Argentina. Bolletino del Museo Regionale di Scienze Naturali Torino 11:137–199
Lobo F, Espinoza RE, Quinteros S (2010) A critical review and systematic discussion of recent classification proposals for liolaemus lizards. Zootaxa 2549:1–30
Meirmans PG, Van Tienderen PH (2004) GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Mol Ecol Notes 4:792–794
Raymond M, Rousset F (1995) GENEPOP version 1.2. Population genetics software for exact tests and ecuminicism. J Hered 86:248–249
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgments
Support was provided by the NSF “Partnership for International Research and Education” award (OISE 0530267) for collaborative research on Patagonian biodiversity, granted to the following institutions (listed alphabetically): BYU, Centro Nacional Patagónico, Dalhousie University, Darwinion Botanical Institute, George Washington University, Universidad Nacional de Cordoba, Universidad Austral de Chile, Universidad Nacional del Comahue, and the Universidad de Concepción. Additional funds were provided by grants from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, PIP), and Fondo Nacional de Ciencia y Tecnica (FONCYT, PICT) issued to L.J. Avila and M. Morando. We also thank the BYU Office of Research and Creative Activities (ORCA) for support of undergraduate student research.
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Hanna, N., Brown, D., Avila, L.J. et al. Characterization of 10 polymorphic microsatellite loci in the South American lizard Liolaemus fitzingerii with cross-amplification in L. chehuachekenk . Conservation Genet Resour 4, 105–107 (2012). https://doi.org/10.1007/s12686-011-9485-5
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DOI: https://doi.org/10.1007/s12686-011-9485-5