Abstract
We developed ten polymorphic microsatellite markers for two species of freshwater anomura endemic to Chile, Aegla araucaniensis and Aegla pewenchae, using two PCR-based methods, PIMA and ISSR-PCR. Nine markers per species gave useful PCR products, revealing moderate to high levels of genetic variation. The number of alleles per locus ranged from 2 to 13 and the observed heterozygosity from 0.103 to 0.929. All the markers appeared to segregate independently. No departure from Hardy–Weinberg equilibrium was found in A. araucaniensis while three markers showed evidence for deficits of heterozygotes and presence of null alleles in A. pewenchae. These markers will be useful for population genetics analyses.
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Acknowledgments
We thank Ivan Vera, Andrea Hueche and Paloma Gachon for technical assistance, and Ricardo Galleguillos, Cristian Gallardo Escárate and Eduardo Ruiz Ponce for providing the RAPD and ISSR primers. This research was funded by a grant from the Chilean National Research Agency FONDECYT #1090404. NG was also supported by the project INNOVA-CORFO #06FC01IBC-71.
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Gouin, N., Bórquez, J., Samollow, P.B. et al. Isolation of microsatellite markers for two Chilean freshwater anomuran species (Aegla araucaniensis and Aegla pewenchae) using PCR-based methods. Conservation Genet Resour 4, 93–96 (2012). https://doi.org/10.1007/s12686-011-9482-8
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DOI: https://doi.org/10.1007/s12686-011-9482-8