Isolation of microsatellite loci from the millipede, Brachycybe nodulosa Verhoeff
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Brachycybe nodulosa is a fungivorous millipede that distributed in Japan and Korea. This species shows patchy distributions and the exclusive paternal care of eggs, and thus is an important subject for both conservation and evolutionary biology. Thirteen polymorphic microsatellite loci were newly isolated from the taxon. Moderate to high levels of polymorphism were observed, with numbers of alleles ranged from 2 to 10. The mean observed and expected heterozygosities ranged from 0.200 to 1.000 and 0.405 to 0.886, respectively. Two loci showed lower heterozygosities than the expectation under HWE. No linkage disequilibrium was detected for any pair of the loci. These new loci will be useful in conservation genetics and evolutionary ecology studies of this species.
KeywordsMicrosatellite Millipede Genetics Paternal care
We thank Ryuji Yasuo and Chiharu Koshio for their assistance in sampling specimens. This study was partly supported by the Grant-in-Aids for Scientific Research of Japan Society for the Promotion of Science (KAKENHI 17570022 to SK and KAKENHI 20370030 to EH).
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