Conservation Genetics Resources

, Volume 4, Issue 1, pp 89–91

Isolation of microsatellite loci from the millipede, Brachycybe nodulosa Verhoeff

  • Eisuke Hasegawa
  • Izumi Yao
  • Kyoko Futami
  • Norihiro Yagi
  • Kazuya Kobayashi
  • Shin-ichi Kudo
Technical Note
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Abstract

Brachycybe nodulosa is a fungivorous millipede that distributed in Japan and Korea. This species shows patchy distributions and the exclusive paternal care of eggs, and thus is an important subject for both conservation and evolutionary biology. Thirteen polymorphic microsatellite loci were newly isolated from the taxon. Moderate to high levels of polymorphism were observed, with numbers of alleles ranged from 2 to 10. The mean observed and expected heterozygosities ranged from 0.200 to 1.000 and 0.405 to 0.886, respectively. Two loci showed lower heterozygosities than the expectation under HWE. No linkage disequilibrium was detected for any pair of the loci. These new loci will be useful in conservation genetics and evolutionary ecology studies of this species.

Keywords

Microsatellite Millipede Genetics Paternal care 

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Eisuke Hasegawa
    • 1
  • Izumi Yao
    • 2
  • Kyoko Futami
    • 2
  • Norihiro Yagi
    • 1
  • Kazuya Kobayashi
    • 1
  • Shin-ichi Kudo
    • 3
  1. 1.Laboratory of Animal Ecology, Department of Ecology and Systematics, Graduate School of AgricultureHokkaido UniversitySapporoJapan
  2. 2.Laboratory of Systematic Entomology, Department of Ecology and Systematics, Graduate School of AgricultureHokkaido UniversitySapporoJapan
  3. 3.Department of BiologyNaruto University of EducationNarutoJapan

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