Abstract
Twenty-three polymorphic microsatellite markers for Sympetrum frequens were developed and characterized. The number of distinct alleles per locus in 32 individuals ranged from 2 to 23. The observed heterozygosity ranged from 0.031 to 0.938, while the expected heterozygosity varied from 0.031 to 0.922. No loci deviated significantly from Hardy–Weinberg equilibrium, no linkage disequilibrium was observed between pairs of loci and no loci showed evidence of null alleles. These microsatellite markers are expected to contribute to future investigations of genetic variation and structure in S. frequens populations.
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Acknowledgments
This study was supported in part by a Grant-in-Aid for Scientific Research (C-20580270, C-23580340 and B-22380133) from the Japan Society for the Promotion of Science. The first author would like to thank Mses. K. Yamanoi and P. Goto for analysis support.
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Koizumi, N., Quinn, T.W., Jinguji, H. et al. Development and characterization of 23 polymorphic microsatellite markers for Sympetrum frequens . Conservation Genet Resour 4, 67–70 (2012). https://doi.org/10.1007/s12686-011-9476-6
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DOI: https://doi.org/10.1007/s12686-011-9476-6