A set of miniSTRs for population genetic analyses of tigers (Panthera tigris) with cross-species amplification for seven other Felidae
With ~3500 wild individuals restricted to 7% of their historical range, tigers are now a global conservation symbol. Here we describe eight miniSTR loci for the Indian tiger (Panthera tigris tigris). These loci were polymorphic (average number of alleles 6.75 (SD 1.39)) with high mean observed heterozygosity (0.43 (SD 0.1)). At least six of these loci could be successfully amplified from seven other feline species present in the Indian subcontinent. These miniSTRs could potentially help population genetic analyses of other elusive and endangered felids.
KeywordsPanthera tigris Non-invasive samples miniSTR Felids Population genetics
We would like to thank members of the Ramakrishnan lab for their support, Shomita Mukherjee for small cat samples and Sonia for sequencing. SM is supported by the Department of Science and Technology and PT by the Council for Scientific and Industrial Research Graduate Fellowship. National Centre for Biological Sciences and Department of Science and Technology, Government of India provided funding for this work.
- Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinfo Online 1:47–50Google Scholar
- Sanderson E, Forrest J, Loucks C et al. (2006) Setting priorities for the conservation and recovery of wild tigers: 2005–2015, The technical assessment. Available: http://www.worldwildlife.org/species/finder/tigers/publications.html