Abstract
A set of 12 single nucleotide polymorphisms selected for both confirming Salmo trutta identity and distinguishing the six European evolutionary lineages were included in a multiplex single-base primer extension assay to confidently assign brown trout ancient DNA to one of the six European haplogroups. The assignment rate reaches 75% for brown trout scales collected 40 years ago.
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Acknowledgments
The authors are indebted to the numerous collaborators that kindly provided us with Salmo samples: E. Lerceteau-Köhler and S. Weiss from Austria, L. Bernatchez from Canada, A. Champigneulle, P. Davaine, E. Beall, J. Le Gentil, R. Guyomard, F. Lange, S. Launey, P. Berrebi, and J. Rives from France, A. Apostolidis from Greece, A. Was and R. Wenne from Poland, J. Dannewitz from Sweden, and E. Garcia–Vazquez M. López-Villar and P. Martinez from Spain. This work was supported by grants from INRA (“Projet innovant EFPA 2008”) and from CNRS (Zone Atelier “Terres Australes et Subantartiques”). We thank M.-F. Deguilloux, O. Lepais, N. Merchermek, C. Tentelier, and three anonymous reviewers for comments.
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Chat, J., Manicki, A. A SNP-based assay in a non model species: evolutionary lineage assignment of brown trout ancient DNA. Conservation Genet Resour 4, 59–62 (2012). https://doi.org/10.1007/s12686-011-9474-8
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DOI: https://doi.org/10.1007/s12686-011-9474-8