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Isolation and characterization of microsatellite loci in the federally endangered fat threeridge mussel (Amblema neislerii)

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Abstract

We isolated and characterize 16 microsatellite loci from Amblema neislerii, a federally endangered freshwater mussel. Fifteen of 16 loci were polymorphic with an average of 11.62 alleles per locus and an average observed and expected heterozygosity of 0.63 and 0.75, respectively. Fourteen of 16 loci conformed to Hardy–Weinberg expectations and all pairs of loci showed no significant genotypic disequilibrium. These microsatellite loci will serve as important tools in ongoing conservation efforts of A. neislerii.

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Acknowledgments

We thank S. Abbott, B. Dudely, K. Herrington, N. Rankin and S. Pursifull, for collection of mussel samples and Genetic Identification Services for construction and sequencing of enriched microsatellite libraries.

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Authors and Affiliations

  1. Department of Biology, University of Florida, 223 Bartram Hall, Gainesville, FL, 32611, USA

    E. Díaz-Ferguson

  2. Warm Springs Fish Technology Center, Conservation Genetics Laboratory, US Fish and Wildlife Service, 5308 Spring Street, Warm Springs, GA, 31830, USA

    A. S. Williams & G. R. Moyer

Authors
  1. E. Díaz-Ferguson
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  2. A. S. Williams
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  3. G. R. Moyer
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Correspondence to E. Díaz-Ferguson.

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Díaz-Ferguson, E., Williams, A.S. & Moyer, G.R. Isolation and characterization of microsatellite loci in the federally endangered fat threeridge mussel (Amblema neislerii). Conservation Genet Resour 3, 757–759 (2011). https://doi.org/10.1007/s12686-011-9451-2

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  • DOI: https://doi.org/10.1007/s12686-011-9451-2

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