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Conservation Genetics Resources

, Volume 3, Issue 3, pp 585–587 | Cite as

Ten polymorphic microsatellite loci for the Atlantic Silverside, Menidia menidia

  • Elizabeth J. Sbrocco
  • Paul H. Barber
Technical Note

Abstract

Ten polymorphic microsatellite loci were isolated from the Atlantic Silverside (Menidia menidia) in order to test hypotheses regarding the role of adaptive phenotypic variation in structuring estuarine populations along coastal North America. Loci were amplified in three multiplex panels requiring a total of four individual PCRs. All loci were highly polymorphic in individuals screened from an estuary in Nova Scotia, and none exhibited significant departures from Hardy–Weinberg equilibrium. The results suggest that these loci will be sensitive to low levels of neutral divergence among populations across M. menidia populations.

Keywords

Atherinidae Menidia menidia Microsatellites Northwest Atlantic Silverside 

Notes

Acknowledgments

We thank David Conover, Lyndie Hice, and Tara Duffy for providing M. menidia samples. Funding was provided by NSF BioOce 0425728.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Department of BiologyBoston UniversityBostonUSA
  2. 2.Department of Ecology and Evolutionary BiologyUniversity of California Los AngelesLos AngelesUSA

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