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Development of microsatellite markers for a diving duck, the common pochard (Aythya ferina)

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Abstract

Ten polymorphic microsatellites for the common pochard were isolated from microsatellite enriched libraries. Seven microsatellites were obtained based on cross-species amplification. These 17 microsatellites exhibited polymorphism within a population sample, with numbers of alleles per locus ranging from 2 to 17 and expected heterozygosities from 0.053 to 0.916. These markers will be helpful for studying reproductive strategies in the pochard, as well as in addressing population genetic questions. Four of the microsatellite loci showed significant departures from Hardy–Weinberg equilibrium and/or high estimates of null allele frequencies in our study population. Hence, they should be used with caution.

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Acknowledgments

We thank David Hardekopf for valuable comments. This research was supported by Grant no. 0021620828 of the Ministry of Education of the Czech Republic, by research aim of AS CR no. AV0Z60930519 and by Charles University GA UK no. 56007. T.A. and J.K. were supported by Research Centrum project no. LC06073, VAV grant SP2D3-60-08 and the grant GAAV KJB601110803. Y.L. and G.H. were supported by a grant from the Swiss Veterinary Office (BVET). M.V. was funded by the by Research Project of the Masaryk University, Brno, Project no.: MSM 0021622 416 and partially supported by the Ichthyoparasitology Centre of Excellence Project no. LC 522, funded by Ministry of Education, Youth and Sports of the CR.

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Authors and Affiliations

  1. Department of Zoology, Faculty of Science, Charles University in Prague, Viničná 7, 12844, Praha 2, Czech Republic

    Ondřej Št’ovíček, Tomáš Albrecht & Jakub Kreisinger

  2. Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic v.v.i, Květná 8, 60365, Brno, Czech Republic

    Dagmar Čížková & Tomáš Albrecht

  3. Computational and Molecular Population Genetics (CMPG), Institute of Ecology and Evolution, University of Bern, 3012, Bern, Switzerland

    Yang Liu & Gerald Heckel

  4. Swiss Institute of Bioinformatics, 1015, Lausanne, Switzerland

    Yang Liu & Gerald Heckel

  5. Institute of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 61137, Brno, Czech Republic

    Martina Vyskočilová

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  1. Ondřej Št’ovíček
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  2. Dagmar Čížková
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  3. Yang Liu
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  4. Tomáš Albrecht
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  5. Gerald Heckel
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  6. Martina Vyskočilová
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  7. Jakub Kreisinger
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Correspondence to Jakub Kreisinger.

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Št’ovíček, O., Čížková, D., Liu, Y. et al. Development of microsatellite markers for a diving duck, the common pochard (Aythya ferina). Conservation Genet Resour 3, 573–576 (2011). https://doi.org/10.1007/s12686-011-9407-6

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  • DOI: https://doi.org/10.1007/s12686-011-9407-6

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