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Development and characterization of nine microsatellite loci for the endangered Kidneyshell, Ptychobranchus fasciolaris, and cross-amplification in closely-related lampsilines (Bivalvia: Unionoida)

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Abstract

Nine polymorphic microsatellite loci were isolated and characterized for the unionid Ptychobranchus fasciolaris. Screening of individuals from two Ontario rivers detected 4–15 alleles per locus and within-population heterozygosities from 0.280 to 0.800. Cross-amplification tests in six other lampsiline species largely yielded polymorphic data, suggesting that these loci should prove useful in population assessments for other species as well.

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Acknowledgments

Funding was provided by the ESRF of World Wildlife Fund Canada, Fisheries and Oceans Canada, and Ontario Ministry of Natural Resources. Friedrich Fischer, Ryan Hill, and Kirsty Hill assisted with field sampling. Missouri State University, University of Alabama Museum and Auburn University Museum provided tissue samples.

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Author notes

  1. Heather S. Galbraith

    Present address: US Geological Survey, Northern Appalachian Research Laboratory, Wellsboro, PA, 16901, USA

Authors and Affiliations

  1. Biology Department, Trent University, 2140 East Bank Drive, Peterborough, ON, K9J 7B8, Canada

    Heather S. Galbraith

  2. Natural Resources DNA Profiling and Forensics Centre, Trent University, 2140 East Bank Drive, Peterborough, ON, K9J 7B8, Canada

    Caleigh M. Smith, Kristyne M. Wozney & Chris C. Wilson

  3. Aquatic Research and Development Section, Ontario Ministry of Natural Resources, Trent University, 2140 East Bank Drive, Peterborough, ON, K9J 7B8, Canada

    Caleigh M. Smith, Kristyne M. Wozney & Chris C. Wilson

  4. Biology Department, Central Michigan University, Mount Pleasant, MI, 48859, USA

    David T. Zanatta

Authors
  1. Heather S. Galbraith
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  2. Caleigh M. Smith
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  3. Kristyne M. Wozney
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  4. David T. Zanatta
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Correspondence to Heather S. Galbraith.

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Galbraith, H.S., Smith, C.M., Wozney, K.M. et al. Development and characterization of nine microsatellite loci for the endangered Kidneyshell, Ptychobranchus fasciolaris, and cross-amplification in closely-related lampsilines (Bivalvia: Unionoida). Conservation Genet Resour 3, 533–536 (2011). https://doi.org/10.1007/s12686-011-9397-4

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  • DOI: https://doi.org/10.1007/s12686-011-9397-4

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