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Isolation and characterization of polymorphic microsatellite loci from Salminus hilarii (Characiformes: Characidae)

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Abstract

Salminus hilarii is a carnivorous fish species which inhabits different drainages in the Neotropical region. Despite the absence of this species in the commercial freshwater fishery, it plays an important role in the food chain, keeping the ecological balance in the freshwater ecosystems. We isolated 9 polymorphic microsatellite markers with alleles varying between 6 and 17 per locus in a test population of 20 individuals. Two loci (Sh49 and Sh65) showed significant departure from Hardy–Weinberg equilibrium (P < 0.05). No significant linkage disequilibrium between pairs of loci was found. These loci will provide useful molecular markers to assess genetic variability of wild and captive populations used in stocking programs.

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Acknowledgments

This study was supported by grant of FAEP (Fundação de Apoio ao Ensino e a Pesquisa). This work is a part of the Master’s Thesis in Biotechnology at the University of Mogi das Cruzes.

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Authors and Affiliations

  1. Unit of Biotechnology, Laboratory of Aquatic Organism Genetics and Aquaculture, University of Mogi das Cruzes, CP 411, Mogi das Cruzes, SP, 08701-970, Brazil

    Juliana Viana Silva & Alexandre Wagner Silva Hilsdorf

  2. Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Av. Dr. Cândido Xavier de Almeida Souza 200, Mogi das Cruzes, SP, 08780-911, Brazil

    Alexandre Wagner Silva Hilsdorf

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  1. Juliana Viana Silva
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  2. Alexandre Wagner Silva Hilsdorf
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Correspondence to Alexandre Wagner Silva Hilsdorf.

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Silva, J.V., Hilsdorf, A.W.S. Isolation and characterization of polymorphic microsatellite loci from Salminus hilarii (Characiformes: Characidae). Conservation Genet Resour 3, 437–439 (2011). https://doi.org/10.1007/s12686-010-9374-3

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  • DOI: https://doi.org/10.1007/s12686-010-9374-3

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