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Rapid development of microsatellites for the endangered Neotropical catfish Conorhynchus conirostris using a modest amount of 454 shot-gun pyrosequencing

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Abstract

A set of 13 polymorphic microsatellites were developed using a next generation sequencing approach for the endangered Neotropical catfish Conorhynchus conirostris. Using only 1/16 of a shot-gun pyrosequencing reaction, we were able to obtain 3,796 sequences containing putative microsatellites motifs. Out of 20 selected loci for further optimization, 13 loci were successfully genotyped in 25 specimens from a locality in the São Francisco River, Southeast Brazil. All microsatellite loci were polymorphic, with 4–18 alleles per locus (mean = 10.5) and generally high values of heterozygosity (mean = 0.796). These polymorphic markers will be a valuable tool for captive breeding and stoking programs and for analyses of population connectivity and genetic structure in this endangered Neotropical catfish.

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Acknowledgments

We would like to thank José Vanderval (IEF) and Francisco Andrade (CEMIG) for helping with the fieldwork, Mike Gardner for assistance with data analysis and Minami Sasaki and Mariana Bertelli for laboratory assistance. DC is grateful to CAPES, an institution from the Brazilian Ministry of Education, for a postdoctoral fellowship (# 4095-09-0). This work was supported by funds from Flinders University to LBB.

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  1. Molecular Ecology Laboratory, School of Biological Sciences, Flinders University, Adelaide, SA, 5001, Australia

    Daniel C. Carvalho & Luciano B. Beheregaray

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  1. Daniel C. Carvalho
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  2. Luciano B. Beheregaray
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Correspondence to Daniel C. Carvalho.

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Carvalho, D.C., Beheregaray, L.B. Rapid development of microsatellites for the endangered Neotropical catfish Conorhynchus conirostris using a modest amount of 454 shot-gun pyrosequencing. Conservation Genet Resour 3, 373–375 (2011). https://doi.org/10.1007/s12686-010-9365-4

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  • DOI: https://doi.org/10.1007/s12686-010-9365-4

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