Abstract
To investigate intraspecific variation and hybridisation in the Lepidosperma costale species complex, forty-one primers to amplify regions containing chloroplast microsatellites and insertions/deletions were designed using de novo sequencing. Twenty-six loci were polymorphic within three diploid populations, with 2 to 9 alleles per locus. A further 15 loci exhibited fixed size differences between the diploid individuals and a population of putative allopolyploid origin. These markers will assist studies of genetic diversity and hybridisation in Lepidosperma, help inform the conservation of taxa in the Lepidosperma costale species complex, and may have utility across the Cyperaceae.
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Acknowledgments
We thank Rod Peakall for providing the set of universal primers and Yinglong Chen for technical assistance. This research was funded by Gindalbie Metals Ltd. MW and RLB were supported by Australian Postgraduate Awards.
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Wallace, M.J., Barrett, M.D. & Barrett, R.L. Novel chloroplast markers for the study of intraspecific variation and hybridisation in the Lepidosperma costale species complex (Cyperaceae). Conservation Genet Resour 3, 355–360 (2011). https://doi.org/10.1007/s12686-010-9361-8
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DOI: https://doi.org/10.1007/s12686-010-9361-8