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Characterization of ten microsatellite loci in the Broad-tailed Hummingbird (Selasphorus platycercus)

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Abstract

The Broad-tailed Hummingbird (Selaphorus platycercus) breeds at higher elevations in the central and southern Rockies, eastern California, and Mexico and has been studied for 8 years in Rocky Mountain National Park, Colorado. Questions regarding the relatedness of Broad-tailed Hummingbirds banded together and then recaptured in close time proximity in later years led us to isolate and develop primers for 10 polymorphic microsatellite loci. In a screen of 25 individuals from a population in Rocky Mountain National Park, the 10 loci were found to have levels of variability ranging from two to 16 alleles. No loci were found to depart from linkage disequilibrium, although two loci revealed significant departures from Hardy–Weinberg equilibrium. These 10 microsatellite loci will be applicable for population genetic analyses, investigation of mating systems and relatedness, and may help gain insight into the migration timing and routes for this species.

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Acknowledgments

This work was funded by the National Park Service. The use of any trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. We thank J. St John for her technical help on this project and H. Earnest and J. Ruth for helpful comments on this manuscript.

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Correspondence to Sara J. Oyler-McCance.

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Oyler-McCance, S.J., Fike, J.A., Talley-Farnham, T. et al. Characterization of ten microsatellite loci in the Broad-tailed Hummingbird (Selasphorus platycercus). Conservation Genet Resour 3, 351–353 (2011). https://doi.org/10.1007/s12686-010-9360-9

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  • DOI: https://doi.org/10.1007/s12686-010-9360-9

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