Conservation Genetics Resources

, Volume 3, Issue 2, pp 345–350 | Cite as

Primers for novel microsatellite markers in “fire-specialist” lizards (Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus) and their performance across multiple populations

  • Annabel L. Smith
  • Michael G. Gardner
  • C. Michael Bull
  • Don A. Driscoll
Technical Note


We developed 45 microsatellite markers for three lizard species with fire-related distributions: Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus (17, 12 and 16 markers respectively). To isolate microsatellites we used an enrichment technique for N. stellatus and next-generation sequencing for A. norrisi and C. atlas. Fluorescent tags were attached to primers during PCR for flexible genotyping. All loci were polymorphic with 2–24 alleles and expected heterozygosities of 0.043–0.927. These markers will facilitate studies of post-fire dispersal and recolonisation.


Dispersal Fire ecology Gene flow Population genetics Reptiles 



This research was supported by funds from the Australian Research Council and the Government of South Australia’s Wildlife Conservation Fund. Thanks for technical support go to Kathy Saint, Alison Fitch, Steve Donnellan and Terry Bertozzi at the South Australian Museum.


  1. Bonin A, Bellemain E, Bronken Eidesen P, Pompanon F, Brochmann C, Taberlet P (2004) How to track and assess genotyping errors in population genetics studies. Mol Ecol 13:3261–3273CrossRefPubMedGoogle Scholar
  2. Clarke MF (2008) Catering for the needs of fauna in fire management: science or just wishful thinking? Wildl. Res 35:385–394CrossRefGoogle Scholar
  3. DeWoody J, Nason JD, Hipkins VD (2006) Mitigating scoring errors in microsatellite data from wild populations. Mol Ecol Notes 6:951–957CrossRefGoogle Scholar
  4. Driscoll DA, Henderson MK (2008) How many common reptile species are fire specialists? A replicated natural experiment highlights the predictive weakness of a fire succession model. Biol Conserv 141:460–471CrossRefGoogle Scholar
  5. Gardner MG, Cooper SJB, Bull CM, Grant WN (1999) Isolation of microsatellite loci from a social lizard, Egernia stokesii, using a modified enrichment procedure. J Hered 90:301–304CrossRefGoogle Scholar
  6. Gardner MG, Sanshez JJ, Dudaniec RY, Rheinberger L, Smith AL, Saint KM (2008) Tiliqua rugosa microsatellites: isolation via enrichment and characterisation of loci for multiplex PCR in T. rugosa and the endangered T. adelaidensis. Conserv Genet 9:233–237CrossRefGoogle Scholar
  7. Hayden MJ, Nguyen TM, Waterman A, Chalmers KJ (2008) Multiplex-Ready PCR: a new method for multiplexed SSR and SNP genotyping. BMC Genomics 9:80CrossRefPubMedGoogle Scholar
  8. Hochberg Y (1988) A sharper Bonferroni procedure for multiple tests of significance. Biometrika 75:800–802CrossRefGoogle Scholar
  9. Meglécz E (2007) MICROFAMILY (version 1): a computer program for detecting flanking-region similarities among different microsatellite loci. Mol Ecol Notes 7:18–20CrossRefGoogle Scholar
  10. Meglécz E, Costedoat C, Debut V, Gilles A, Malausa T, Pech N, Martin J-F (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26(3):403–404CrossRefPubMedGoogle Scholar
  11. Murphy AR, Gardner MG, Fox SF (2009) Isolation of microsatellites via enrichment and a sequence tagged method in a South American lizard with suspected parental care, Liolaemus leopardinus. Conserv Genet Resour 1(1):13–16. doi: 10.1007/s12686-009-9003-1 CrossRefGoogle Scholar
  12. Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295CrossRefGoogle Scholar
  13. Raymond M, Rousset F (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  14. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  15. Whelan RJ, Rodgerson L, Dickman CR, Sutherland EF (2002) Critical life cycles of plants and animals: developing a process-based understanding of population changes in fire-prone landscapes. In: Bradstock RA, Williams JE, Gill AM (eds) Flammable Australia: the fire regimes and biodiversity of a continent. Cambridge University Press, CambridgeGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Annabel L. Smith
    • 1
  • Michael G. Gardner
    • 2
  • C. Michael Bull
    • 2
  • Don A. Driscoll
    • 1
  1. 1.Fenner School of Environment and SocietyThe Australian National UniversityCanberraAustralia
  2. 2.School of Biological SciencesFlinders UniversityAdelaideAustralia

Personalised recommendations