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Conservation Genetics Resources

, Volume 3, Issue 2, pp 345–350 | Cite as

Primers for novel microsatellite markers in “fire-specialist” lizards (Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus) and their performance across multiple populations

  • Annabel L. Smith
  • Michael G. Gardner
  • C. Michael Bull
  • Don A. Driscoll
Technical Note

Abstract

We developed 45 microsatellite markers for three lizard species with fire-related distributions: Amphibolurus norrisi, Ctenotus atlas and Nephrurus stellatus (17, 12 and 16 markers respectively). To isolate microsatellites we used an enrichment technique for N. stellatus and next-generation sequencing for A. norrisi and C. atlas. Fluorescent tags were attached to primers during PCR for flexible genotyping. All loci were polymorphic with 2–24 alleles and expected heterozygosities of 0.043–0.927. These markers will facilitate studies of post-fire dispersal and recolonisation.

Keywords

Dispersal Fire ecology Gene flow Population genetics Reptiles 

Notes

Acknowledgments

This research was supported by funds from the Australian Research Council and the Government of South Australia’s Wildlife Conservation Fund. Thanks for technical support go to Kathy Saint, Alison Fitch, Steve Donnellan and Terry Bertozzi at the South Australian Museum.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Annabel L. Smith
    • 1
  • Michael G. Gardner
    • 2
  • C. Michael Bull
    • 2
  • Don A. Driscoll
    • 1
  1. 1.Fenner School of Environment and SocietyThe Australian National UniversityCanberraAustralia
  2. 2.School of Biological SciencesFlinders UniversityAdelaideAustralia

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