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Isolation and characterization of polymorphic microsatellite markers for the ariake icefish (Salanx ariakensis kishinouye)

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Abstract

Twenty microsatellite markers were isolated and characterized in the ariake icefish (Salanx ariakensis kishinouye). Analysis of 28 individuals from a single population revealed an average of 17.75 alleles per locus, which ranged from 8 to 26. The mean observed and expected heterozygosity (H O and H E) were 0.8410 and 0.9262, respectively. The average polymorphism information content was 0.8973, ranging from 0.7824 to 0.9521. These newly isolated markers will be useful in analyzing genetic diversity and genetic relations between the ariake icefish and its relatives.

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Acknowledgments

The authors would like to thank professor Bao Baolong for his suggestions and comments on the manuscript. This research was supported by the Shanghai Education Committee Foundation (S30701 and 09YZ279), the Natural Science Foundation of Shanghai (No. 09ZR1421700) and the Shanghai Science and Technology Project (No. 10dz1200804).

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Authors and Affiliations

  1. College of Fisheries and Life Science, Shanghai Ocean University, 999 Hucheng Huan Road, 201306, Shanghai, China

    Juan Hu, Sheng-Jie Ren & Xiao-Ling Gong

  2. Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, 200433, Shanghai, China

    Cui-Zhang Fu

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  1. Juan Hu
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  2. Sheng-Jie Ren
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  3. Cui-Zhang Fu
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  4. Xiao-Ling Gong
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Correspondence to Xiao-Ling Gong.

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Hu, J., Ren, SJ., Fu, CZ. et al. Isolation and characterization of polymorphic microsatellite markers for the ariake icefish (Salanx ariakensis kishinouye). Conservation Genet Resour 3, 315–318 (2011). https://doi.org/10.1007/s12686-010-9352-9

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  • DOI: https://doi.org/10.1007/s12686-010-9352-9

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