Abstract
Thirteen polymorphic tri- and tetra-nucleotide microsatellites are reported for the spotted snow skink (Niveoscincus ocellatus) from Tasmania. Variation was assessed among 40 individuals collected from a single locality. Most loci had 12–19 alleles, although one had more than 20 alleles, while observed heterozygosities ranged between 0.25 and 0.93. Eleven loci were also polymorphic in the congeneric species N. metallicus (N = 16 collected from a single locality).
This is a preview of subscription content, log in via an institution to check access.
References
Atkins N, Swain R, Wapstra E, Jones SM (2007) Late stage deferral of parturition in the viviparous lizard Niveoscincus ocellatus (Gray 1845): implications for offspring quality and survival. Biol J Linn Soc 90:735–746
Cadby CD, While GM, Hobday A, Uller T, Wapstra E (2010) The effect of climatic variation at the local, regional and global scale on offspring size and date of birth in a terrestrial reptile. Integr Zool 5:163–174
Jones SM, Swain R (1996) Annual reproductive cycle and annual cycles of reproductive hormones in plasma of female Niveoscincus metallicus (Scincidae) from Tasmania. J Herpetol 30:140–146
Jones SM, Swain R (2006) Placental transfer of 3H- oleic acid in three species of viviparous lizards: A route for supplementation of embryonic fat bodies? Herpetol Monogr 20:186–193
Jones KC, Levine KF, Banks JD (2002) Characterization of 11 polymorphic tetranucleotide microsatellites for forensic applications in California elk (Cervus elaphus canadensis). Mol Ecol Notes 2:425–427
Olsson M, Shine R, Wapstra E, Ujvari B, Madsen T (2002) Sexual dimorphism in lizard body shape: the roles of sexual selection and fecundity selection. Evolution 56:1538–1542
Olsson M, Ujvari B, Wapstra E, Madsen T, Shine R, Bensch S (2005) Does mate guarding prevent rival mating in snow skink lizards? A test using AFLP. Herpetologica 61:389–394
Pen I, Uller T, Feldmeyer B, Harts A, While GM, Wapstra E (2010) Climate driven population divergence in sex determining systems. Nature. doi:10.1038/nature09512
Rice WR (1989) Analysing tables of statistical tests. Evolution 43:223–225
Rousset F (2008) GENEPOP ‘007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resources 8:103–106
Van Oosterhout C (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Wapstra E, Swain R (2001) Geographic and annual variation in life history traits in a small Australian skink. J Herpetol 35:194–203
Wapstra E, Swain R, Jones SM, O’Reilly JM (1999) Geographic and annual variation in the timing of reproductive cycles in a temperate skink, Niveoscincus ocellatus. Aust J Zool 47:539–550
Wapstra E, Swain R, O’Reilly JM (2001) Geographic variation in age and size at maturity in a small Australian viviparous skink. Copeia 2001:646–655
Wapstra E, Olsson M, Shine R, Edwards A, Swain R, Joss JMP (2004) Maternal basking behaviour determines offspring sex in a viviparous reptile. Proc Roy Soc B 271:S230–S232
Wapstra E, Uller T, Sinn DL, Olsson M, Mazurek K, Joss J, Shine R (2009) Climate effects on offspring sex ratio in a viviparous lizard. J Anim Ecol 78:84–90
Wapstra E, Uller T, While G, Olsson M, Shine R (2010) Giving offspring a head start in life: field and experimental evidence for selection on maternal basking behaviour in lizards. J Evol Biol 23:651–657
While GM, Wapstra E (2009) Snow skinks (Niveoscincus ocellatus) do not shift their sex allocation patterns in response to mating history. Behaviour 146:1405–1422
Acknowledgments
The Australian Research Council and the Hermon Slade Foundation provided financial support. Adam Smolenski, Natasha Wiggins and Ashley Edwards assisted during the early stages of the project. Electrophoresis was performed by AGRF (Adelaide), and microsatellite clones were developed by Genetic Identification Services (California).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Worth, J., Burridge, C., While, G. et al. Development of 13 microsatellite loci in the spotted snow skink Niveoscincus ocellatus (Squamata: Scincidae). Conservation Genet Resour 3, 287–290 (2011). https://doi.org/10.1007/s12686-010-9343-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-010-9343-x