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Development and characterization of 18 microsatellite loci for the Southern Leopard Frog, Rana sphenocephala

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Abstract

We isolated and characterized 18 microsatellite loci for the Southern Leopard Frog, Rana sphenocephala. Loci were screened in 30 individuals of R. sphenocephala. The number of alleles per locus ranged from 5 to 21, observed heterozygosity ranged from 0.200 to 0.933, and the probability of identity values ranged from 0.008 to 0.299. These new loci are tools that can be used to study population genetic structure, genetic diversity, and gene flow across varying habitat types and scales.

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Acknowledgments

Manuscript preparation was partially supported by the DOE under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation. We thank David Scott for providing tissue samples from known sex and family groups of R. sphenocephala. We thank Dr. Lora Smith and the herpetology lab at the Jones Ecological Research Center for their assistance collecting R. sphenocephala samples.

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Authors and Affiliations

  1. Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA, 30602, USA

    Anna M. McKee

  2. Department of Environmental Health Science, University of Georgia, Athens, GA, 30602, USA

    Travis C. Glenn

  3. Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC, 29802, USA

    Stacey L. Lance & Cris Hagen

  4. Georgia Genomics Facility, University of Georgia, Athens, GA, 30602, USA

    Kenneth L. Jones & Travis C. Glenn

Authors
  1. Anna M. McKee
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  2. Stacey L. Lance
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  3. Kenneth L. Jones
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  4. Cris Hagen
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  5. Travis C. Glenn
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Correspondence to Anna M. McKee.

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This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof.

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McKee, A.M., Lance, S.L., Jones, K.L. et al. Development and characterization of 18 microsatellite loci for the Southern Leopard Frog, Rana sphenocephala . Conservation Genet Resour 3, 267–269 (2011). https://doi.org/10.1007/s12686-010-9338-7

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  • DOI: https://doi.org/10.1007/s12686-010-9338-7

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